Wild-type (WT) and ERK knockout (KO) 293T cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] detects ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) protein by immunofluorescent analysis.
Sample: Mock and treated A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) stained by ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] detects ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) stained by ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] detects ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) protein at endoplasmic reticulum and cytoplasm by immunofluorescent analysis.
Sample: NIH-3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) stained by ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500 and competitor's antibody (# Highly Cited Antibody) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500 and competitor's antibody (# Highly Cited Antibody) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Untreated (–) and treated (+) A431 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.