ERK2抗体 [6F8]

FACS analysis of HEK293T cells transfected with either ERK2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84154 ERK2 antibody [6F8].

WB analysis of various samples using GTX84154 ERK2 antibody [6F8].
Loading : 10 ug per lane
Dilution : 1:200

WB analysis of HEK293T cells transfected with ERK2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84154 ERK2 antibody [6F8].
Loading : 5 ug per lane

IP analysis of DDDDK tagged ERK2 overexpressed HEK293T lysate using GTX84154 ERK2 antibody [6F8].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

WB analysis of various cell lines using GTX84154 ERK2 antibody [6F8].
Loading : 35 ug per lane

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84154 ERK2 antibody [6F8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human breast adenocarcinoma tissue using GTX84154 ERK2 antibody [6F8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

WB analysis of wild type and ERK2 knock out HeLa cell lysate(10μg per lane) using GTX84154 ERK2 antibody [6F8].
Dilution : 1:500