产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
Observed MW
120 kDa. The observed M.W. is based on the following publication. PMID: 15692064
产品属性
形式
Liquid
存储溶液
PBS, 0.05% BSA, 50% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.77 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
A synthesized peptide derived from human ERK5
纯化方式
Purified by antigen-affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
mitogen-activated protein kinase 7 , BMK1 , ERK4 , ERK5 , PRKM7
细胞定位
Cytoplasm , Nucleus
功能与背景
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855](/templates/default/js/lightbox/images/loading.gif "Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855")
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GTX02855 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERK5 antibody [GT1258] (GTX02855) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 15692064
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GTX02855 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERK5 antibody [GT1258] (GTX02855) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 15692064
|
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GTX02855 IHC-P Image
IHC-P analysis of mouse kidney tissue section using GTX02855 ERK5 antibody [GT1258].
Dilution : 1:100
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GTX02855 IHC-P Image
IHC-P analysis of mouse kidney tissue section using GTX02855 ERK5 antibody [GT1258].
Dilution : 1:100
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GTX02855 WB Image
WB analysis of various samples using GTX02855 ERK5 antibody [GT1258].
Dilution : 1:500
Loading : 25μg
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GTX02855 IHC-P Image
IHC-P analysis of human colon tissue section using GTX02855 ERK5 antibody [GT1258].
Dilution : 1:100
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GTX02855 WB Image
WB analysis of various samples using GTX02855 ERK5 antibody [GT1258].
Dilution : 1:500
Loading : 25μg
|
文献引用
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产品评论
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![Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855](/upload/website/prouct_img/small/GTX02855/GTX02855_4000000871_20210122_WB_M_R_w_23053123_881.webp "Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855")
![Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855](/upload/website/prouct_img/small/GTX02855/GTX02855_4000000871_20210122_WB_w_23053123_944.webp "Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855")
![Anti-ERK5 antibody [GT1258] used in IHC (Paraffin sections) (IHC-P). GTX02855](/upload/website/prouct_img/small/GTX02855/A3948_IHC_03_(1071650)_w_23053123_783.webp "Anti-ERK5 antibody [GT1258] used in IHC (Paraffin sections) (IHC-P). GTX02855")
![Anti-ERK5 antibody [GT1258] used in IHC (Paraffin sections) (IHC-P). GTX02855](/upload/website/prouct_img/small/GTX02855/A3948_IHC_02_(1071649)_w_23053123_556.webp "Anti-ERK5 antibody [GT1258] used in IHC (Paraffin sections) (IHC-P). GTX02855")
![Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855](/upload/website/prouct_img/small/GTX02855/CutImage_A3948_WB_01_(1070252)_w_23053123_689.webp "Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855")
![Anti-ERK5 antibody [GT1258] used in IHC (Paraffin sections) (IHC-P). GTX02855](/upload/website/prouct_img/small/GTX02855/A3948_IHC_01_(1071648)_w_23053123_570.webp "Anti-ERK5 antibody [GT1258] used in IHC (Paraffin sections) (IHC-P). GTX02855")
![Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855](/upload/website/prouct_img/small/GTX02855/CutImage_A3948_WB_02_(1070253)_w_23053123_130.webp "Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855")
![Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855](/upload/website/prouct_img/normal/GTX02855/GTX02855_4000000871_20210122_WB_M_R_w_23053123_881.webp "Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855")
![Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855](/upload/website/prouct_img/normal/GTX02855/GTX02855_4000000871_20210122_WB_w_23053123_944.webp "Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855")
![Anti-ERK5 antibody [GT1258] used in IHC (Paraffin sections) (IHC-P). GTX02855](/upload/website/prouct_img/normal/GTX02855/A3948_IHC_03_(1071650)_w_23053123_783.webp "Anti-ERK5 antibody [GT1258] used in IHC (Paraffin sections) (IHC-P). GTX02855")
![Anti-ERK5 antibody [GT1258] used in IHC (Paraffin sections) (IHC-P). GTX02855](/upload/website/prouct_img/normal/GTX02855/A3948_IHC_02_(1071649)_w_23053123_556.webp "Anti-ERK5 antibody [GT1258] used in IHC (Paraffin sections) (IHC-P). GTX02855")
![Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855](/upload/website/prouct_img/normal/GTX02855/CutImage_A3948_WB_01_(1070252)_w_23053123_689.webp "Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855")
![Anti-ERK5 antibody [GT1258] used in IHC (Paraffin sections) (IHC-P). GTX02855](/upload/website/prouct_img/normal/GTX02855/A3948_IHC_01_(1071648)_w_23053123_570.webp "Anti-ERK5 antibody [GT1258] used in IHC (Paraffin sections) (IHC-P). GTX02855")
![Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855](/upload/website/prouct_img/normal/GTX02855/CutImage_A3948_WB_02_(1070253)_w_23053123_130.webp "Anti-ERK5 antibody [GT1258] used in Western Blot (WB). GTX02855")
