产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HepG2 , HCT116 , Neuro2A , GL261 , C8D30 , 293T , A431 , HeLa , PC-12 , Rat2
产品说明
Knockdown/Knockout & overexpression validation were supported by customer review data.
预测种属反应
Rabbit, Sheep, Bovine, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human ERp57. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_10720538
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
protein disulfide isomerase family A member 3 , ER60 , ERp57 , ERp60 , ERp61 , GRP57 , GRP58 , HEL-S-269 , HEL-S-93n , HsT17083 , P58 , PI-PLC
细胞定位
Endoplasmic reticulum lumen , Melanosome
功能与背景
This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX113719 ICC/IF Image
ERp57 antibody detects ERp57 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERp57 protein stained by ERp57 antibody (GTX113719) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
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GTX113719 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with ERp57 antibody (GTX113719) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX113719 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody (GTX113719) diluted at 1:500.
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GTX113719 WB Image
ERp57 antibody detects ERp57 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERp57 antibody (GTX113719) diluted by 1:10000.
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GTX113719 WB Image
Sample (30 ug of whole cell lysate)
A: HepG2
B: HCT116
7.5% SDS PAGE
GTX113719 diluted at 1:10000
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GTX113719 IHC-P Image
ERp57 antibody detects ERp57 protein at cytoplasm on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
ERp57 antibody (GTX113719) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113719 IHC-P Image
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ERp57(GTX113719) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113719 WB Image
Sample (30 ug of whole cell lysate)
A: Neuro2A
B: GL261
C: C8D30
7.5% SDS PAGE
GTX113719 diluted at 1:1000
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文献引用
产品评论
ERp57 antibody
目录号 GTX113719
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Rating
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( Average 4 based on 2 users reviews)
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实验应用
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Western Blot(WB)
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( Average 4 based on 2 users reviews)
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Date :
Anonymous submitted on 02-Nov-2018
Application Tested :
WB
Sample Species :
Hu
Sample :
293T, HeLa cells
Amount used :
10 μg
Blocking :
Blocking One, 25°C, 1Hr
Primary Antibody :
1:500, 4°C, 16Hr
Date :
Anonymous submitted on 27-Nov-2015
Application Tested :
WB
Sample Species :
Hu
Sample :
Caco2 cells
Amount used :
20μg
Blocking :
5% milk in PBST, 25°C, 1.5Hr
Primary Antibody :
1:1000, 4°C, 16Hr
