摘要
EasyBlot anti-Goat IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of IgG and does not bind to the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP).
The protein A/G beads used in the IP procedure sometimes bind to IgG and produce contaminating signals at approximately 50 kDa and 25 kDa. This kit also includes an EasyBlocker (GTX425858) that can minimize the background caused by Protein A/G contamination. GeneTex’s EasyBlot kit is simple to use and detects only the target band in your IP experiments, resulting in cleaner western blots.
Content
| EasyBlot Kit Package |
Content |
Package |
Storage Temp |
| GTX228910-01 (250μl) |
GTX628547-01 EasyBlot anti-Goat IgG (HRP) |
250μl |
-20 ºC |
| GTX425858 EasyBlocker |
60g |
4 ºC |
| GTX228910-01 (50μl) |
GTX628547-01 EasyBlot anti-Goat IgG (HRP) |
50μl |
-20 ºC |
| GTX425858 EasyBlocker |
12g |
4 ºC |
|
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.18 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Goat
Target Isotype
IgG
纯化方式
Affinity purified by Protein G.
偶联
Horseradish peroxidase(HRP)
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
