Non-transfected (–) and transfected (+) 293T whole cell extracts (5 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Ebola virus VP35 antibody (GTX134032) diluted at 1:5000 and DDDDK tag antibody (GTX115043) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Ebola virus VP35 antibody detects Ebola virus VP35 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ebola virus VP35 protein stained by Ebola virus VP35 antibody (GTX134032) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.