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Enterovirus 71 3C抗体 [B3]

Overexpression
Anti-Enterovirus 71 3C antibody [B3] used in Western Blot (WB). GTX630191
Anti-Enterovirus 71 3C antibody [B3] used in Western Blot (WB). GTX630191
Anti-Enterovirus 71 3C antibody [B3] used in Western Blot (WB). GTX630191
Anti-Enterovirus 71 3C antibody [B3] used in Western Blot (WB). GTX630191

目录号 GTX630191

文献引用 9

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 B3

同种型

 IgG1

实验应用

 WB, FACS

种属反应

 Coxsackievirus A6, Enterovirus 71
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Enterovirus 71 3C 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
FACS Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

阳性对照

EV71 C2-infected RD , CVA6-infected RD

产品说明

This antibody is raised against Enterovirus 71, and it may cross react with Coxsackievirus A6 based on internal testing.

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Enterovirus 71

免疫原

The immunogen used to generate this antibody corresponds to EV71 3C

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888195

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Enterovirus 71 Protease 3C , Enterovirus 71 3C , EV71 Protease 3C , EV71 3C

研究领域

產品图片

Anti-Enterovirus 71 3C antibody [B3] used in Western Blot (WB). GTX630191

GTX630191 WB Image

Non-infected (–) and infected (+) RD whole cell extracts (20 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 3C antibody [B3] (GTX630191) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Enterovirus 71 3C antibody [B3] used in Western Blot (WB). GTX630191

GTX630191 WB Image

Non-infected (–) and infected (+) RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 3C antibody [B3] (GTX630191) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Enterovirus 71 3C antibody [B3] used in Western Blot (WB). GTX630191

GTX630191 WB Image

Overexpression

The data was published in the journal PLoS Pathog in 2017.PMID: 28985237

Anti-Enterovirus 71 3C antibody [B3] used in Western Blot (WB). GTX630191

GTX630191 WB Image

The data was published in the journal PLoS Negl Trop Dis in 2015.PMID: 25855976

文献引用

Application Reference

J Han et al. PLoS Pathog 2024; Microtubule disruption synergizes with STING signaling to show potent and broad-spectrum antiviral activity
Kang NX et al. Acta Pharmacol Sin 2022; 43 (4) : 977-991 Anemoside B4 inhibits enterovirus 71 propagation in mice through upregulating 14-3-3 expression and type I interferon responses.
实验应用 : WB
Fan W et al. J Virol 2022; 96 (19) : e0133222 Enterovirus 3C Protease Cleaves TRIM7 To Dampen Its Antiviral Activity.
Zhao X et al. J Virol 2022; 96 (6) : e0011922 N-Acetyltransferase 8 Promotes Viral Replication by Increasing the Stability of Enterovirus 71 Nonstructural Proteins.
Arthur Huang KY et al. Nat Commun 2017; 8 (1) : 762 Epitope-associated and specificity-focused features of EV71-neutralizing antibody repertoires from plasmablasts of infected children.
实验应用 : FACS
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Application Reference
J Han et al. PLoS Pathog 2024; Microtubule disruption synergizes with STING signaling to show potent and broad-spectrum antiviral activity
Kang NX et al. Acta Pharmacol Sin 2022; 43 (4):977-991 Anemoside B4 inhibits enterovirus 71 propagation in mice through upregulating 14-3-3 expression and type I interferon responses.
实验应用 : WB
Fan W et al. J Virol 2022; 96 (19):e0133222 Enterovirus 3C Protease Cleaves TRIM7 To Dampen Its Antiviral Activity.
Zhao X et al. J Virol 2022; 96 (6):e0011922 N-Acetyltransferase 8 Promotes Viral Replication by Increasing the Stability of Enterovirus 71 Nonstructural Proteins.
Wang T et al. PLoS Pathog 2017; 13 (10):e1006674 Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.
实验应用 : WB
Arthur Huang KY et al. Nat Commun 2017; 8 (1):762 Epitope-associated and specificity-focused features of EV71-neutralizing antibody repertoires from plasmablasts of infected children.
实验应用 : FACS
Kim C et al. Virol J 2016; 13 (1):99 Antiviral activity of micafungin against enterovirus 71.
实验应用 : WB
Jui-Chung Wu et al. Sci Rep 2015; 5 (0):18476 Focal adhesion kinase-dependent focal adhesion recruitment of SH2 domains directs SRC into focal adhesions to regulate cell adhesion and migration.
实验应用 : WB
Tsou YL et al. PLoS Negl Trop Dis 2015; 9 (4):e0003692 Recombinant Adeno-Vaccine Expressing Enterovirus 71-Like Particles against Hand, Foot, and Mouth Disease.
实验应用 : WB
SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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