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Enterovirus 71 VP1抗体 [GT185]

Orthogonal
Anti-Enterovirus 71 VP1 antibody [GT185] used in ELISA (ELISA). GTX633390
Anti-Enterovirus 71 VP1 antibody [GT185] used in Western Blot (WB). GTX633390
Anti-Enterovirus 71 VP1 antibody [GT185] used in Western Blot (WB). GTX633390
Anti-Enterovirus 71 VP1 antibody [GT185] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633390

目录号 GTX633390

文献引用 1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT185

同种型

 IgG1

实验应用

 WB, ICC/IF, ELISA

种属反应

 Enterovirus 71
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Enterovirus 71 VP1 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
ELISA Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品说明

This antibody was raised against Enterovirus 71 VP1, and it does not cross-react with Enterovirus D68 VP1.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Enterovirus 71

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Enterovirus 71 VP1 (EV71 virus (strain USA/BrCr/1970)). The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888339

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Enterovirus 71 Capsid protein VP1 , Enterovirus 71 VP1 , EV71 Capsid protein VP1 , EV71 VP1

研究领域

產品图片

Anti-Enterovirus 71 VP1 antibody [GT185] used in ELISA (ELISA). GTX633390

GTX633390 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Enterovirus 71 VP1 protein, His tag (GTX138560-pro) (4000-62.5 ng/mL). Coated protein was probed with Enterovirus 71 VP1 antibody [GT185] (GTX633390) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.

Anti-Enterovirus 71 VP1 antibody [GT185] used in Western Blot (WB). GTX633390

GTX633390 WB Image

Orthogonal

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [GT185] (GTX633390) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Enterovirus 71 VP1 antibody [GT185] used in Western Blot (WB). GTX633390

GTX633390 WB Image

Non-infected (–) and infected (+) RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [GT185] (GTX633390) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Enterovirus 71 VP1 antibody [GT185] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633390

GTX633390 ICC/IF Image

Enterovirus 71 VP1 antibody [GT185] detects Enterovirus 71 VP1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Enterovirus 71 VP1 stained by Enterovirus 71 VP1 antibody [GT185] (GTX633390) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

文献引用

Application Reference

Wang Y et al. J Med Virol 2023; 95 (1) : e28400 Phosphorylation of enteroviral 2A(pro) at Ser/Thr125 benefits its proteolytic activity and viral pathogenesis.
实验应用 : WB
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PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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