EpCAM抗体 [GT25512]

Name: EpCAM抗体 [GT25512]
Brand: GeneTex
SKU: GTX635967
Price: 4000.00 RMB

目录号 GTX635967

GTX635967 FCM Image

HCT116 (EpCAM positive), HeLa (EpCAM negative)

EpCAM antibody[GT25512] (GTX635967) detects EpCAM by flow cytometry analysis.
Sample: HeLa and HCT-116
Blue: Mouse IgG isotype control.
Red: EpCAM antibody[GT25512] (GTX635967) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.
HCT116 (EpCAM positive), HeLa (EpCAM negative)

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GTX635967 ICC/IF Image

EpCAM antibody [GT25512] detects EpCAM protein at cell membrane by immunofluorescent analysis.
Sample: HCT116 and HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by EpCAM antibody [GT25512] (GTX635967) diluted at 1:500.

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GTX635967 IHC-P Image

EpCAM antibody [GT25512] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded MDA-MB-231 xenograft.
EpCAM stained by EpCAM antibody [GT25512] (GTX635967) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX635967 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with EpCAM antibody [GT25512] (GTX635967) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX2131

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宿主

Mouse
克隆

Monoclonal
克隆编号

GT25512
同种型

IgG2a
实验应用

WB ICC/IF IHC-P FCM
种属反应

Human

摘要

EpCAM antibody recognizes epithelial cell adhesion molecule (EpCAM), a transmembrane glycoprotein with a predicted molecular weight of ~35 kDa. EpCAM is upregulated in epithelial cells during epithelial–mesenchymal transition (EMT) and is used as a diagnostic marker of many carcinomas and circulating tumor cells. Detection of EpCAM by immunohistochemistry is important for the diagnosis of certain epithelial malignancies.