产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
Negative Control
HeLa
产品说明
This antibody was raised against human EpCAM Extracellular domain.
我们不建议将此产品应用于 Mouse samples.
预测种属反应
Mouse, Rat, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the Extracellular domain of human EpCAM. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2909925
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1
功能与背景
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
数据库
研究领域
產品图片
![Anti-EpCAM antibody [GT3188] used in IHC (Paraffin sections) (IHC-P). GTX635970](/templates/default/js/lightbox/images/loading.gif "Anti-EpCAM antibody [GT3188] used in IHC (Paraffin sections) (IHC-P). GTX635970")
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GTX635970 IHC-P Image
EpCAM antibody [GT3188] detects EpCAM protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded MDA-MB-231 xenograft.EpCAM stained by EpCAM antibody [GT3188] (GTX635970) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635970 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EpCAM antibody[GT3188] (GTX635970) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX635970 FACS Image
EpCAM antibody[GT3188] (GTX635970) detects EpCAM by flow cytometry analysis.
Sample: HeLa and HCT-116
Blue: Mouse IgG isotype control.
Red: EpCAM antibody[GT3188] (GTX635970) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.
HCT116 (EpCAM positive), HeLa (EpCAM negative)
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GTX635970 ICC/IF Image
EpCAM antibody [GT3188] detects EpCAM protein at cell membrane by immunofluorescent analysis.Sample: HCT116 and HeLa cells were fixed in ice-cold MeOH for 5 min.Green: EpCAM stained by EpCAM antibody [GT3188] (GTX635970) diluted at 1:500.
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文献引用
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产品评论
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![Anti-EpCAM antibody [GT3188] used in IHC (Paraffin sections) (IHC-P). GTX635970](/upload/website/prouct_img/small/GTX635970/GTX635970_44216_20210326_IHC-P_w_23061202_241.webp "Anti-EpCAM antibody [GT3188] used in IHC (Paraffin sections) (IHC-P). GTX635970")
![Anti-EpCAM antibody [GT3188] used in Western Blot (WB). GTX635970](/upload/website/prouct_img/small/GTX635970/GTX635970_44216_20210219_WB_w_23061202_552.webp "Anti-EpCAM antibody [GT3188] used in Western Blot (WB). GTX635970")
![Anti-EpCAM antibody [GT3188] used in Flow cytometry (FACS). GTX635970](/upload/website/prouct_img/small/GTX635970/GTX635970_44216_20210226_FACS_w_23061202_575.webp "Anti-EpCAM antibody [GT3188] used in Flow cytometry (FACS). GTX635970")
![Anti-EpCAM antibody [GT3188] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635970](/upload/website/prouct_img/small/GTX635970/GTX635970_44216_20210219_ICC_IF_w_23061202_425.webp "Anti-EpCAM antibody [GT3188] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635970")
![Anti-EpCAM antibody [GT3188] used in IHC (Paraffin sections) (IHC-P). GTX635970](/upload/website/prouct_img/normal/GTX635970/GTX635970_44216_20210326_IHC-P_w_23061202_241.webp "Anti-EpCAM antibody [GT3188] used in IHC (Paraffin sections) (IHC-P). GTX635970")
![Anti-EpCAM antibody [GT3188] used in Western Blot (WB). GTX635970](/upload/website/prouct_img/normal/GTX635970/GTX635970_44216_20210219_WB_w_23061202_552.webp "Anti-EpCAM antibody [GT3188] used in Western Blot (WB). GTX635970")
![Anti-EpCAM antibody [GT3188] used in Flow cytometry (FACS). GTX635970](/upload/website/prouct_img/normal/GTX635970/GTX635970_44216_20210226_FACS_w_23061202_575.webp "Anti-EpCAM antibody [GT3188] used in Flow cytometry (FACS). GTX635970")
![Anti-EpCAM antibody [GT3188] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635970](/upload/website/prouct_img/normal/GTX635970/GTX635970_44216_20210219_ICC_IF_w_23061202_425.webp "Anti-EpCAM antibody [GT3188] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635970")
