产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
Assay dependent |
Assay dependent |
1:100-1:1000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Rat, Sheep, Bovine, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human EpCAM. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2910067
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1
功能与背景
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
数据库
研究领域
產品图片
|
GTX636758 FACS Image
EpCAM antibody [HL1338] (GTX636758) detects EpCAM protein by flow cytometry analysis. Sample: HCT-116 and HeLa cell. Gray: Rabbit IgG Isotype control. Red: EpCAM antibody [HL1338] (GTX636758) dilution: 1:50. Acquisition of 50,000 events were collected for flow cytometry analysis.
|
|
GTX636758 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EpCAM antibody [HL1338] (GTX636758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX636758 IHC-P Image
EpCAM antibody [HL1338] detects EpCAM protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human HCT116 xenograft. EpCAM stained by EpCAM antibody [HL1338] (GTX636758) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX636758 ICC/IF Image
EpCAM antibody [HL1338] detects EpCAM protein at cell membrane and cell junction by immunofluorescent analysis. Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min. Green: EpCAM stained by EpCAM antibody [HL1338] (GTX636758) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
|
|
GTX636758 IHC-P Image
EpCAM antibody [HL1338] detects EpCAM protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.EpCAM stained by EpCAM antibody [HL1338] (GTX636758) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX636758 IHC-P Image
EpCAM antibody [HL1338] detects EpCAM protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. EpCAM stained by EpCAM antibody [HL1338] (GTX636758) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
文献引用
目前没有使用 EpCAM antibody [HL1338] (GTX636758) 的文献被收录。快来回报我们第一篇参考文献吧!
产品评论
目前没有使用 EpCAM antibody [HL1338] (GTX636758) 的产品评论被收录。快发表第一篇产品评论吧!