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EpCAM抗体 [HL1339]

Recombinant antibody Orthogonal
Anti-EpCAM antibody [HL1339] used in Western Blot (WB). GTX636759
Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759
Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759
Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759
Anti-EpCAM antibody [HL1339] used in Flow cytometry (FACS). GTX636759
Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759
Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759
Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759

目录号 GTX636759

文献引用 1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1339

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, FACS

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

35 kDa. ( Note )

预测种属反应

Sheep, Bovine, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human EpCAM. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2910068

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1

功能与背景

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

数据库

研究领域

產品图片

Anti-EpCAM antibody [HL1339] used in Western Blot (WB). GTX636759

GTX636759 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EpCAM antibody [HL1339] (GTX636759) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759

GTX636759 IHC-P Image

EpCAM antibody [HL1339] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759

GTX636759 IHC-P Image

EpCAM antibody [HL1339] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human HCT116 xenograft.
EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759

GTX636759 IHC-P Image

EpCAM antibody [HL1339] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EpCAM antibody [HL1339] used in Flow cytometry (FACS). GTX636759

GTX636759 FACS Image

Orthogonal

EpCAM antibody [HL1339] (GTX636759) detects EpCAM protein by flow cytometry analysis.
Sample: HCT-116 and HeLa cell.
Gray: Rabbit IgG Isotype control.
Red: EpCAM antibody [HL1339] (GTX636759) dilution: 1:50.
Acquisition of 50,000 events were collected for flow cytometry analysis.

Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759

GTX636759 IHC-P Image

EpCAM antibody [HL1339] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759

GTX636759 ICC/IF Image

EpCAM antibody [HL1339] detects EpCAM protein at cell membrane and cell junction by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759

GTX636759 ICC/IF Image

EpCAM antibody [HL1339] detects EpCAM protein at cell membrane and cell junction by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

文献引用

Application Reference

P Bisaccia et al. Stem Cells Transl Med 2023; : szad070 Extracellular Vesicles From Mesenchymal Umbilical Cord Cells Exert Protection Against Oxidative Stress and Fibrosis in a Rat Model of Bronchopulmonary Dysplasia
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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