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EpCAM抗体 [MSVA-326R] HistoMAX™

HistoMAX
Anti-EpCAM antibody [MSVA-326R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04380
Anti-EpCAM antibody [MSVA-326R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04380
Anti-EpCAM antibody [MSVA-326R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04380

目录号 GTX04380

宿主

Rabbit

克隆

Monoclonal

克隆编号

MSVA-326R

同种型

IgG

实验应用

IHC-P

种属反应

Human
产品包装
500 μl (¥7300),
100 μl (¥4200)
产品说明

摘要

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, the pH 9.0 showed a strongest staining pattern. The pH 7.8 is acceptable but lower pH results in a significant reduction of sensitivity.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

35 kDa. ( Note )

阳性对照

Kidney: In kidney, distal tubule cells must show a strong predominantly membranous staining, while at a least moderate predominantly basolateral staining must be seen in the majority of proximal tubules cells and in scattered epithelial cells lining the Bowman capsule.

Negative Control

Tonsil: EpCAM staining should be absent in lymphocytes or smooth muscle cells of the vessels.

产品属性

形式

Liquid

存储溶液

PBS, 0.05% BSA (Please contact us for PBS only format)

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100-224)

纯化方式

Protein A/G purified

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1

细胞定位

Cell junction, tight junction

功能与背景

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

数据库

研究领域

產品图片

Anti-EpCAM antibody [MSVA-326R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04380

GTX04380 IHC-P Image

IHC-P analysis of human lung tissue section using GTX04380 EpCAM antibody [MSVA-326R] HistoMAX™.
Strong EPCAM staining in an adenocarcinoma of the lung.

Anti-EpCAM antibody [MSVA-326R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04380

GTX04380 IHC-P Image

IHC-P analysis of human epitheloid malignant mesothelioma tissue section using GTX04380 EpCAM antibody [MSVA-326R] HistoMAX™.
EPCAM immunostaining is lacking in an epitheloid malignant mesothelioma.

Anti-EpCAM antibody [MSVA-326R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04380

GTX04380 IHC-P Image

IHC-P analysis of human kidney tissue section using GTX04380 EpCAM antibody [MSVA-326R] HistoMAX™.
In normal kidney, EpCAM staining is strong in the distal tubules and moderate in the proximal tubulus predominantly basolateral and in epithelial cells lining the Bowman capsule.

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产品包装 报价 (¥)
¥ 7300
¥ 4200
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