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Estrogen Receptor alpha抗体 [1F3]

Comparison Orthogonal Overexpression
Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171
Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171
Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171
Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

目录号 GTX70171

文献引用 7

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 1F3

同种型

 IgG1

实验应用

 WB, IHC-Fr, FACS, IP

种属反应

 Human, Rabbit
产品包装
100 μl (¥4300)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-Fr Assay dependent
FACS Assay dependent
IP 1 - 5 μg/ml
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

66 kDa. ( Note )

阳性对照

MCF-7 , DDDDK-tagged ESR1-transfected 293T

产品说明

This antibody is specific for ER alpha protein, and it does not cross react with ER beta protein.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.56 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

The region encoding amino acids 1-190 expressed in E. coli.

纯化方式

Protein G purified
From tissue culture supernatant

偶联

Unconjugated

RRID

AB_370361

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1

细胞定位

Cytoplasm , Cell membrane , Cell membrane; Single-pass type I membrane protein , Golgi apparatus

功能与背景

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

数据库

研究领域

產品图片

Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

GTX70171 WB Image

Whole cell extract (60 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody (GTX70171) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate (GTX14698).

Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

GTX70171 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [1F3] (GTX70171) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

GTX70171 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [1F3] (GTX70171) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The observed expression level is based on the publication: PMID: 24724101

Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

GTX70171 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Estrogen Receptor alpha antibody [1F3] (GTX70171) diluted at 1:500 and competitor's antibody (MA1-310) diluted at 1:100. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.
The observed M.W. is based on the publication: PMID: 26397740

文献引用

Application Reference

Chang YC et al. Front Cell Dev Biol 2020; 8 : 485 Tamoxifen Rechallenge Decreases Metastatic Potential but Increases Cell Viability and Clonogenicity in a Tamoxifen-Mediated Cytotoxicity-Resistant Subline of Human Breast MCF7 Cancer Cells.
实验应用 : WB
种属反应 : Human
Ma L et al. Lung Cancer Manag 2019; 8 (1) : LMT04 Targeting claudin-3 suppresses stem cell-like phenotype in nonsquamous non-small-cell lung carcinoma.
Daurio NA et al. Cancer Res 2016; (Epub) ampk activation and metabolic reprogramming by * through estrogen receptor-independent mechanisms suggests new uses for this therapeutic modality in cancer treatment.
实验应用 : WB
种属反应 : Human
Mahajan K et al. J Biol Chem 2014; 289 (41) : 28179-91 ACK1 tyrosine kinase interacts with histone demethylase KDM3A to regulate the mammary tumor oncogene HOXA1.
实验应用 : WB
种属反应 : Human
Ivanova MM et al. Mol Cell Endocrinol 2009; 305 (43102) : 43455 Activity and intracellular location of estrogen receptors alpha and beta in human bronchial epithelial cells.
实验应用 : WB
种属反应 : Human
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Application Reference
Chang YC et al. Front Cell Dev Biol 2020; 8:485 Tamoxifen Rechallenge Decreases Metastatic Potential but Increases Cell Viability and Clonogenicity in a Tamoxifen-Mediated Cytotoxicity-Resistant Subline of Human Breast MCF7 Cancer Cells.
实验应用 : WB
种属反应 : Human
Ma L et al. Lung Cancer Manag 2019; 8 (1):LMT04 Targeting claudin-3 suppresses stem cell-like phenotype in nonsquamous non-small-cell lung carcinoma.
Daurio NA et al. Cancer Res 2016; (Epub) ampk activation and metabolic reprogramming by * through estrogen receptor-independent mechanisms suggests new uses for this therapeutic modality in cancer treatment.
实验应用 : WB
种属反应 : Human
Mahajan K et al. J Biol Chem 2014; 289 (41):28179-91 ACK1 tyrosine kinase interacts with histone demethylase KDM3A to regulate the mammary tumor oncogene HOXA1.
实验应用 : WB
种属反应 : Human
Ivanova MM et al. Mol Cell Endocrinol 2009; 305 (43102):43455 Activity and intracellular location of estrogen receptors alpha and beta in human bronchial epithelial cells.
实验应用 : WB
种属反应 : Human
Srilatha B et al. Urology 2004; 63 (2):382-6 Estrogen and phytoestrogen predispose to erectile dysfunction: do ER-alpha and ER-beta in the cavernosum play a role?
实验应用 : IHC-Fr
种属反应 : Rabbit
Clone Reference
Sun M et al. Mol Cancer Ther 2008; 7 (3):464-73 Curcumin (diferuloylmethane) alters the expression profiles of microRNAs in human pancreatic cancer cells.
实验应用 : FACS
种属反应 : Human
SDS
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产品包装 报价 (¥)
¥ 4300
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