产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for ER alpha protein, and it does not cross react with ER beta protein.
预测种属反应
Mouse, Rat, Bovine, Cat, Dog, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the N-terminus region of human Estrogen Receptor alpha. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2909953
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1
细胞定位
Cytoplasm,Cell membrane,Cell membrane; Single-pass type I membrane protein,Golgi apparatus
功能与背景
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]
数据库
研究领域
產品图片
![Anti-Estrogen Receptor alpha antibody [HL1082] used in Western Blot (WB). GTX636203](/templates/default/js/lightbox/images/loading.gif "Anti-Estrogen Receptor alpha antibody [HL1082] used in Western Blot (WB). GTX636203")
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GTX636203 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Estrogen Receptor alpha antibody [HL1082] (GTX636203) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636203 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [HL1082] (GTX636203) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636203 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [HL1082] (GTX636203) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636203 IHC-P Image
Estrogen Receptor alpha antibody [HL1082] detects Estrogen Receptor alpha protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Estrogen Receptor alpha stained by Estrogen Receptor alpha antibody [HL1082] (GTX636203) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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![Anti-Estrogen Receptor alpha antibody [HL1082] used in Western Blot (WB). GTX636203](/upload/website/prouct_img/small/GTX636203/GTX636203_44382_20220812_WB_competitor_watermark_22081423_456.webp "Anti-Estrogen Receptor alpha antibody [HL1082] used in Western Blot (WB). GTX636203")
![Anti-Estrogen Receptor alpha antibody [HL1082] used in Western Blot (WB). GTX636203](/upload/website/prouct_img/small/GTX636203/GTX636203_44382_20210730_WB_B_w_23061202_936.webp "Anti-Estrogen Receptor alpha antibody [HL1082] used in Western Blot (WB). GTX636203")
![Anti-Estrogen Receptor alpha antibody [HL1082] used in Western Blot (WB). GTX636203](/upload/website/prouct_img/small/GTX636203/GTX636203_44382_20210723_WB_w_23061202_713.webp "Anti-Estrogen Receptor alpha antibody [HL1082] used in Western Blot (WB). GTX636203")
![Anti-Estrogen Receptor alpha antibody [HL1082] used in IHC (Paraffin sections) (IHC-P). GTX636203](/upload/website/prouct_img/small/GTX636203/GTX636203_44382_20210813_IHC-P_w_23061202_287.webp "Anti-Estrogen Receptor alpha antibody [HL1082] used in IHC (Paraffin sections) (IHC-P). GTX636203")
![Anti-Estrogen Receptor alpha antibody [HL1082] used in Western Blot (WB). GTX636203](/upload/website/prouct_img/normal/GTX636203/GTX636203_44382_20220812_WB_competitor_watermark_22081423_456.webp "Anti-Estrogen Receptor alpha antibody [HL1082] used in Western Blot (WB). GTX636203")
![Anti-Estrogen Receptor alpha antibody [HL1082] used in Western Blot (WB). GTX636203](/upload/website/prouct_img/normal/GTX636203/GTX636203_44382_20210730_WB_B_w_23061202_936.webp "Anti-Estrogen Receptor alpha antibody [HL1082] used in Western Blot (WB). GTX636203")
![Anti-Estrogen Receptor alpha antibody [HL1082] used in Western Blot (WB). GTX636203](/upload/website/prouct_img/normal/GTX636203/GTX636203_44382_20210723_WB_w_23061202_713.webp "Anti-Estrogen Receptor alpha antibody [HL1082] used in Western Blot (WB). GTX636203")
![Anti-Estrogen Receptor alpha antibody [HL1082] used in IHC (Paraffin sections) (IHC-P). GTX636203](/upload/website/prouct_img/normal/GTX636203/GTX636203_44382_20210813_IHC-P_w_23061202_287.webp "Anti-Estrogen Receptor alpha antibody [HL1082] used in IHC (Paraffin sections) (IHC-P). GTX636203")
