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Estrogen Receptor alpha抗体 [MSVA-564R] HistoMAX™

Comparison Orthogonal HistoMAX
Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474
Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474
Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474

目录号 GTX04474

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 MSVA-564R

同种型

 IgG

实验应用

 IHC-P

种属反应

 Human
产品包装
500 μl (¥11100),
100 μl (¥4200)
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产品说明

摘要

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48 and BOND RX - Leica. The detailed steps by Autostainer please refer to the protocol.
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

66 kDa. ( Note )

阳性对照

Uterine cervix: virtually all squamous and endocervical cells must show a moderate to strong nuclear staining. Tonsil: Scattered follicular dendritic cells in germinal centers and some squamous epithelial cells in tonsil crypts must show an at least weak nuclear ER immunostaining.

Negative Control

Tonsil: B-cells in mantle zones and within germinal centers must be negative.

产品属性

形式

Liquid

存储溶液

PBS, 0.05% BSA (Please contact us for PBS only format)

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant fragment (around aa495-595) of human ER alpha protein

纯化方式

Protein A/G purified

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1

细胞定位

Nucleus , Cytoplasm , Cell membrane , Cell membrane; Single-pass type I membrane protein , Nucleus , Golgi apparatus

功能与背景

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

数据库

研究领域

產品图片

Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474

GTX04474 IHC-P Image

IHC-P analysis of human uterus endometrium tissue section using GTX04474 Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™.
Among normal tissues highest levels of Estrogen Receptor alpha immunostaining occur in epithelial and stromal cells of the endometrium.

Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474

GTX04474 IHC-P Image

IHC-P analysis of human uterus cervix tissue section using GTX04474 Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™.
An intense Estrogen Receptor alpha staining is seen in stromal cells and in the majority of squamous epithelial cells uf the uterine ectocervix.

Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474

GTX04474 IHC-P Image

IHC-P analysis of human endometroid ovarian carcinoma tissue section using GTX04474 Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™.
Endometroid carcinoma of the ovary with strong Estrogen Receptor alpha immunostaining of all tumor cells.

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产品包装 报价 (¥)
¥ 11100
¥ 4200
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