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Estrogen Receptor beta抗体 [7B10.7]

Anti-Estrogen Receptor beta antibody [7B10.7] used in Western Blot (WB). GTX70182

目录号 GTX70182

文献引用 19

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 7B10.7

同种型

 IgG1

实验应用

 WB, IP, ChIP assay

种属反应

 Human
产品包装
100 μl (¥4300)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1 - 5 μg/ml
IP Assay dependent
ChIP assay Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

59 kDa. ( Note )

阳性对照

Myc-DDK-tagged ESR2-transfected 293T , Recombinant fusion protein

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Amino acids 1-153 of human ER-§ expressed in E. coli.

纯化方式

Protein G purified
From tissue culture supernatant

偶联

Unconjugated

RRID

AB_370375

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

estrogen receptor 2 , ER-BETA , ESR-BETA , ESRB , ESTRB , Erb , NR3A2 , ODG8

细胞定位

Nucleus

功能与背景

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-Estrogen Receptor beta antibody [7B10.7] used in Western Blot (WB). GTX70182

GTX70182 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [7B10.7] (GTX70182) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Sellitto A et al. Cancers (Basel) 2020; 12 (6) Insights into the Role of Estrogen Receptor β in Triple-Negative Breast Cancer.
Lu W et al. Horm Cancer 2017; (Epub) Estrogen Receptor-β Modulation of the ERα-p53 Loop Regulating Gene Expression, Proliferation, and Apoptosis in Breast Cancer.
实验应用 : ChIP assay
种属反应 : Human
Monsivais D et al. Fertil Steril 2016; 105 (5) : 1266-1273 Estrogen receptor β regulates endometriotic cell survival through serum and glucocorticoid-regulated kinase activation.
实验应用 : IP
种属反应 : Human
Nelson AW et al. Mol Cell Endocrinol 2016; 440 : 138-150 Comprehensive assessment of estrogen receptor beta antibodies in cancer cell line models and tissue reveals critical limitations in reagent specificity.
实验应用 : WB
种属反应 : Human
Monsivais D et al. Mol Endocrinol 2014; : me20131421 ERβ- and Prostaglandin E2-Regulated Pathways Integrate Cell Proliferation via Ras-like and Estrogen Regulated Growth Inhibitor in Endometriosis.
实验应用 : ChIP assay
种属反应 : Human
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Application Reference
Sellitto A et al. Cancers (Basel) 2020; 12 (6) Insights into the Role of Estrogen Receptor β in Triple-Negative Breast Cancer.
Lu W et al. Horm Cancer 2017; (Epub) Estrogen Receptor-β Modulation of the ERα-p53 Loop Regulating Gene Expression, Proliferation, and Apoptosis in Breast Cancer.
实验应用 : ChIP assay
种属反应 : Human
Monsivais D et al. Fertil Steril 2016; 105 (5):1266-1273 Estrogen receptor β regulates endometriotic cell survival through serum and glucocorticoid-regulated kinase activation.
实验应用 : IP
种属反应 : Human
Nelson AW et al. Mol Cell Endocrinol 2016; 440:138-150 Comprehensive assessment of estrogen receptor beta antibodies in cancer cell line models and tissue reveals critical limitations in reagent specificity.
实验应用 : WB
种属反应 : Human
Monsivais D et al. Mol Endocrinol 2014;:me20131421 ERβ- and Prostaglandin E2-Regulated Pathways Integrate Cell Proliferation via Ras-like and Estrogen Regulated Growth Inhibitor in Endometriosis.
实验应用 : ChIP assay
种属反应 : Human
Ruddy SC et al. Mol Cancer Ther 2014; Preferential estrogen receptor β ligands reduce Bcl-2 expression in hormone-resistant breast cancer cells to increase autophagy.
实验应用 : ChIP assay
种属反应 : Human
Madak-Erdogan Z et al. Mol Syst Biol 2013; 9:676 Integrative genomics of gene and metabolic regulation by estrogen receptors α and β, and their coregulators.
实验应用 : ChIP assay
种属反应 : Human
Jiang Y et al. FASEB J 2013; 27 (11):4406-18 Mechanisms enforcing the estrogen receptor β selectivity of botanical estrogens.
实验应用 : ChIP assay
种属反应 : Human
Krishnamurthy N et al. FASEB J 2012; 26 (10):3993-4002 induction of quinone reductase by * or dpn protects against mammary tumorigenesis.
Paruthiyil S et al. Breast Cancer Res Treat 2011; 129 (3):777-84 Estrogen receptor β causes a G2 cell cycle arrest by inhibiting CDK1 activity through the regulation of cyclin B1, GADD45A, and BTG2.
实验应用 : ChIP assay
种属反应 : Human
Vivar OI et al. J Biol Chem 2010; 285 (29):22059-66 Estrogen receptor beta binds to and regulates three distinct classes of target genes.
实验应用 : ChIP assay
种属反应 : Human
Charn TH et al. Mol Endocrinol 2010; 24 (1):47-59 Genome-wide dynamics of chromatin binding of estrogen receptors alpha and beta: mutual restriction and competitive site selection.
实验应用 : ChIP assay
种属反应 : Human
Vivar OI et al. Endocrinology 2010; 151 (4):1662-7 Selective activation of estrogen receptor-beta target genes by 3,3'-diindolylmethane.
实验应用 : ChIP assay
种属反应 : Human
Chang EC et al. Mol Endocrinol 2008; 22 (5):1032-43 Estrogen Receptors alpha and beta as determinants of gene expression: influence of ligand, dose, and chromatin binding.
实验应用 : ChIP assay
种属反应 : Human
Cvoro A et al. Endocrinology 2007; 148 (2):538-47 Selective activation of estrogen receptor-beta transcriptional pathways by an herbal extract.
实验应用 : ChIP assay
种属反应 : Human
Cvoro A et al. Mol Cell 2006; 21 (4):555-64 Distinct roles of unliganded and liganded estrogen receptors in transcriptional repression.
实验应用 : ChIP assay
种属反应 : Human
Tee MK et al. Mol Biol Cell 2004; 15 (3):1262-72 * and selective estrogen receptor modulators differentially regulate target genes with estrogen receptors alpha and beta.
实验应用 : ChIP assay
种属反应 : Human
Bianco NR et al. Mol Endocrinol 2003; 17 (7):1344-55 Functional implications of antiestrogen induction of quinone reductase: inhibition of estrogen-induced deoxyribonucleic acid damage.
实验应用 : WB
种属反应 : Human
Burakov D et al. J Biol Chem 2002; 277 (17):14359-62 Reciprocal recruitment of DRIP/mediator and p160 coactivator complexes in vivo by estrogen receptor.
实验应用 : IP
种属反应 : Human
SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4300
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