产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 0.1-0.3μg/ml |
| 3.75μg/ml |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
The peptide used to generate this antibody is available for purchase (GTX89766-PEP).
产品说明
This antibody is expected to recognise isoform 1 (NP_004449.1) and isoform 2 (NP_075266.1).
预测种属反应
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
TBS, 0.5% BSA
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Peptide with sequence C-HYLKDIERMYGGK, from the C Terminus of the protein sequence according to NP_004449.1; NP_075266.1.
纯化方式
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
acyl-CoA synthetase long chain family member 4 , ACS4 , FACL4 , LACS4 , MRX63 , MRX68
细胞定位
Mitochondrion outer membrane , Endoplasmic reticulum membrane
功能与背景
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq, Jan 2016]
数据库
研究领域
產品图片
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GTX89766 WB Image
WB analysis of HepG2 lysate using GTX89766 FACL4 antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89766 IHC-P Image
IHC-P analysis of human cortex using GTX89766 FACL4 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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GTX89766 IHC-P Image
IHC-P analysis of human small intestine using GTX89766 FACL4 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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