GeneTex中国官方网站
  • China

FACL4抗体 [N1N3]

KO/KD Orthogonal
Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399
Anti-FACL4 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114399
Anti-FACL4 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114399
Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399
Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399
Anti-FACL4 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114399
Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399
Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399
Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399

目录号 GTX114399

文献引用 1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 FACL4 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

79 kDa. ( Note )

阳性对照

HeLa , Raw264.7 , PC-12 , Rat-2 , MCF-7 , MDA-MB-231

预测种属反应

Pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human FACL4. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10619561

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

acyl-CoA synthetase long chain family member 4 , ACS4 , FACL4 , LACS4 , MRX63 , MRX68

细胞定位

Mitochondrion outer membrane; Single-pass type III membrane protein , Peroxisome membrane; Single-pass type III membrane protein , Microsome membrane; Single-pass type III membrane protein , Endoplasmic reticulum membrane; Single-pass type III membrane pr

功能与背景

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399

GTX114399 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (GTX114399) diluted at 1:1000.

Anti-FACL4 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114399

GTX114399 IHC-P Image

FACL4 antibody [N1N3] detects FACL4 protein at cytoplasm in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
FACL4 antibody [N1N3] (GTX114399) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FACL4 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114399

GTX114399 IHC-P Image

FACL4 antibody [N1N3] detects FACL4 protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
FACL4 antibody [N1N3] (GTX114399) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399

GTX114399 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
FACL4 antibody
GTX114399 diluted at 1:1000

Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399

GTX114399 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (GTX114399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FACL4 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114399

GTX114399 ICC/IF Image

FACL4 antibody [N1N3] detects FACL4 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FACL4 protein stained by FACL4 antibody [N1N3] (GTX114399) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399

GTX114399 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (GTX114399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399

GTX114399 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (GTX114399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399

GTX114399 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (GTX114399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Tsai YT et al. Nutrients 2020; 12 (3) Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice.
我要回报文献

产品评论

目前没有使用 FACL4 antibody [N1N3] (GTX114399) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com