Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein by western blot analysis. Left: Untreated (-) and EGF-treated (+, 50 ng/ml for 30 min) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE. Right: Untreated (-) and EGF-treated (+, 200 ng/ml for 30 min) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE. The membranes were blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:3000.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein at cell membrane and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FAK (phospho Tyr576) protein stained by FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
FAK (phospho Tyr576) stained by FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.