GeneTex中国官方网站
  • China

FCER1G抗体 [HL1418]

Recombinant antibody KO/KD Orthogonal Overexpression
Anti-FCER1G antibody [HL1418] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636884
Anti-FCER1G antibody [HL1418] used in Western Blot (WB). GTX636884
Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884
Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884
Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884
Anti-FCER1G antibody [HL1418] used in Western Blot (WB). GTX636884
Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884
Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884

目录号 GTX636884

2

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1418

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human, Mouse, Rat, Cat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 FCER1G 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

10 kDa. ( Note )

产品说明

This antibody was raised against human FCER1G Intracellular domain.

预测种属反应

Bovine, Pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Synthetic peptide encompassing a sequence within the Intracellular domain of human FCER1G. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Fc fragment of IgE receptor Ig , FCRG

细胞定位

Cell membrane

功能与背景

The high affinity IgE receptor is a key molecule involved in allergic reactions. It is a tetramer composed of 1 alpha, 1 beta, and 2 gamma chains. The gamma chains are also subunits of other Fc receptors. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-FCER1G antibody [HL1418] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636884

GTX636884 ICC/IF Image

FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FCER1G stained by FCER1G antibody [HL1418] (GTX636884) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-FCER1G antibody [HL1418] used in Western Blot (WB). GTX636884

GTX636884 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [HL1418] (GTX636884) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884

GTX636884 IHC-P Image

FCER1G antibody [HL1418] detects FCER1G protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
FCER1G stained by FCER1G antibody [HL1418] (GTX636884) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884

GTX636884 IHC-P Image

FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
FCER1G stained by FCER1G antibody [HL1418] (GTX636884) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884

GTX636884 IHC-P Image

FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
FCER1G stained by FCER1G antibody [HL1418] (GTX636884) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FCER1G antibody [HL1418] used in Western Blot (WB). GTX636884

GTX636884 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [HL1418] (GTX636884) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884

GTX636884 IHC-P Image

FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded cat cerebellum.
FCER1G stained by FCER1G antibody [HL1418] (GTX636884) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884

GTX636884 IHC-P Image

FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded dog cerebellum.
FCER1G stained by FCER1G antibody [HL1418] (GTX636884) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

目前没有使用 FCER1G antibody [HL1418] (GTX636884) 的文献被收录。快来回报我们第一篇参考文献吧！
我要回报文献

产品评论

FCER1G antibody [HL1418] 目录号 GTX636884

Rating
( Average 5 based on 2 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-FCER1G antibody [HL1418] used in Western Blot (WB). GTX636884
Date : Anonymous submitted on 24-Apr-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : THP-1 WT and FCER1G KO cells
Amount used : 40 μg
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-FCER1G antibody [HL1418] used in Immunoprecipitation (IP). GTX636884
Date : Anonymous submitted on 24-Apr-2024
Score :
Application Tested : IP
Sample Species : Hu
Sample : THP-1 cells
条带说明 : SM: 6% starting material
UB: 6% unbound fraction
Figure Note : Antibody-bead conjugates were prepared by adding 2.0 μg of antibody to 500 μL of IP Lysis Buffer, together, 30 μL of protein A-Sepharose beads. 0.75 mL aliquots at 0.8 mg/mL of lysate were incubated, an antibody-bead conjugate for ~1 hrs at 4ºC.

Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
我要评论产品
产品包装 报价 (¥)
¥ 4000
¥ 1700
客制化偶联服务
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com