FGFR1 (phospho Tyr766) + FGFR2 (phospho Tyr769) + FGFR3 (phospho Tyr760)抗体 [HL3487]

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FGFR1 (phospho Tyr766) + FGFR2 (phospho Tyr769) + FGFR3 (phospho Tyr760) antibody [HL3487] (GTX641372) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FGFR1 (phospho Tyr766) + FGFR2 (phospho Tyr769) + FGFR3 (phospho Tyr760) antibody [HL3487] (GTX641372) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Specificity test was performed by coating the plate with recombinant FGFR1 non-phospho peptide, FGFR1 (phospho Tyr766) peptide, FGFR2 non-phospho peptide, FGFR2 (phospho Tyr769) peptide, FGFR3 non-phospho peptide, and FGFR3 phospho Tyr760 peptide (250 ng/mL). Coated protein was probed with FGFR1 (phospho Tyr766) + FGFR2 (phospho Tyr769) + FGFR3 (phospho Tyr760) antibody [HL3487] (GTX641372) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.