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FIS1抗体

Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111010
Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111010
Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010
Anti-FIS1 antibody used in Western Blot (WB). GTX111010

目录号 GTX111010

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P

种属反应

Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:20000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

17 kDa. ( Note )

阳性对照

Raji , Mouse brain , mouse liver , 293T , A431 , HeLa , HepG2

预测种属反应

Bovine, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.4 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length human FIS1 Recombinant protein.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950286

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

fission, mitochondrial 1 , CGI-135 , TTC11

细胞定位

Mitochondrion outer membrane; Single-pass membrane protein , Peroxisome membrane; Single-pass membrane protein

功能与背景

The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM]

数据库

研究领域

產品图片

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

Sample (30 μg of whole cell lysate)
A: Raji
15% SDS PAGE
GTX111010 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:10000.

Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111010

GTX111010 IHC-P Image

FIS1 antibody detects FIS1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
FIS1 stained by FIS1 antibody (GTX111010) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111010

GTX111010 IHC-P Image

TTC11 antibody detects TTC11 protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
TTC11 antibody (GTX111010) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111010

GTX111010 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using FIS1(GTX111010) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

FIS1 antibody detects FIS1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX111010 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FIS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111010

GTX111010 ICC/IF Image

FIS1 antibody detects FIS1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: FIS1 protein stained by FIS1 antibody (GTX111010) diluted at 1:2000.
Red: MitoTrackerR Red CMXRos, a mitochondria tracker.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

The data was published in the journal Redox Biol in 2019.PMID: 30388684

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

The data was published in the journal Int J Mol Sci in 2020.PMID: 32560241

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

The data was published in the journal Sci Rep in 2017.PMID: 28220896

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

The data was published in the 2019 in Nat Commun. PMID: 31053718

Anti-FIS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111010

GTX111010 ICC/IF Image

The data was published in the 2019 in Nat Commun. PMID: 31053718

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

The data was published in the 2019 in Nat Commun. PMID: 31053718

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

The data was published in the 2019 in Nat Commun. PMID: 31053718

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

The data was published in the journal FEBS Open Bio in 2019.PMID: 30761259

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

The data was published in the journal Physiol Rep in 2018.PMID: 29932506

Anti-FIS1 antibody used in Western Blot (WB). GTX111010

GTX111010 WB Image

The data was published in the journal PLoS Pathog in 2015.PMID: 26717518

文献引用

产品评论

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Application Reference
Tsu-Kung Lin et al. Front Mol Neurosci 2021; 14:697440
实验应用 : WB
种属反应 : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
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