产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:20000 |
1:100-1:2000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Raji , Mouse brain , mouse liver , 293T , A431 , HeLa , HepG2
预测种属反应
Bovine, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.4 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Full length human FIS1 Recombinant protein.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1950286
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
fission, mitochondrial 1 , CGI-135 , TTC11
细胞定位
Mitochondrion outer membrane; Single-pass membrane protein , Peroxisome membrane; Single-pass membrane protein
功能与背景
The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM]
数据库
研究领域
產品图片
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GTX111010 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111010 WB Image
Sample (30 μg of whole cell lysate) A: Raji 15% SDS PAGE GTX111010 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111010 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:10000.
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GTX111010 IHC-P Image
FIS1 antibody detects FIS1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.FIS1 stained by FIS1 antibody (GTX111010) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX111010 IHC-P Image
TTC11 antibody detects TTC11 protein at mitochondria on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. TTC11 antibody (GTX111010) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX111010 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using FIS1(GTX111010) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX111010 WB Image
FIS1 antibody detects FIS1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111010 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111010 WB Image
Sample (50 μg of whole cell lysate) A: mouse brain 15% SDS PAGE GTX111010 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111010 ICC/IF Image
FIS1 antibody detects FIS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: FIS1 protein stained by FIS1 antibody (GTX111010) diluted at 1:2000. Red: MitoTrackerR Red CMXRos, a mitochondria tracker. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
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GTX111010 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111010 WB Image
The data was published in the journal Redox Biol in 2019.PMID: 30388684
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GTX111010 WB Image
The data was published in the journal Int J Mol Sci in 2020.PMID: 32560241
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GTX111010 WB Image
The data was published in the journal Sci Rep in 2017.PMID: 28220896
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GTX111010 WB Image
The data was published in the 2019 in Nat Commun. PMID: 31053718
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GTX111010 ICC/IF Image
The data was published in the 2019 in Nat Commun. PMID: 31053718
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GTX111010 WB Image
The data was published in the 2019 in Nat Commun. PMID: 31053718
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GTX111010 WB Image
The data was published in the 2019 in Nat Commun. PMID: 31053718
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GTX111010 WB Image
The data was published in the journal FEBS Open Bio in 2019.PMID: 30761259
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GTX111010 WB Image
The data was published in the journal Physiol Rep in 2018.PMID: 29932506
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GTX111010 WB Image
The data was published in the journal PLoS Pathog in 2015.PMID: 26717518
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文献引用
产品评论
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