产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
rat kidney , BCL-1
预测种属反应
Mouse, Bovine, Chicken, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.22 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human FLI1. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_11166318
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
Fli-1 proto-oncogene, ETS transcription factor , BDPLT21 , EWSR2 , SIC-1
细胞定位
Nucleus
功能与背景
Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
数据库
研究领域
產品图片
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GTX112937 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody (GTX112937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112937 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using FLI1(GTX112937) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX112937 ICC/IF Image
FLI1 antibody detects FLI1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FLI1 protein stained by FLI1 antibody (GTX112937) diluted at 1:500.
Blue: Hoechst 33343 staining.
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GTX112937 IP Image
FLI1 antibody immunoprecipitates FLI1 protein in IP experiments.
IP samples: Raji whole cell extract
A. 40 μg Raji whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of FLI1 protein by 4 μg FLI1 antibody (GTX112937)
10 % SDS-PAGE
The immunoprecipitated FLI1 protein was detected by FLI1 antibody (GTX112937) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX112937 WB Image
FLI1 antibody detects FLI1 protein by western blot analysis.
A. 50 μg rat kidney lysate/extract
10% SDS-PAGE
FLI1 antibody (GTX112937) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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