产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HepG2
预测种属反应
Mouse, Rat, Rabbit, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human FMO1. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_10620803
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
flavin containing monooxygenase 1
细胞定位
Microsome membrane , Endoplasmic reticulum membrane
功能与背景
Metabolic N-oxidation of the diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man resulting in a small subpopulation with reduced TMA N-oxidation capacity resulting in fish odor syndrome Trimethylaminuria. Three forms of the enzyme, FMO1 found in fetal liver, FMO2 found in adult liver, and FMO3 are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-FMO1 antibody [N2C1], Internal used in Western Blot (WB). GTX114412](/templates/default/js/lightbox/images/loading.gif "Anti-FMO1 antibody [N2C1], Internal used in Western Blot (WB). GTX114412")
|
GTX114412 WB Image
Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
Flotillin-2 antibody
GTX114412 diluted at 1:1000
|
|
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GTX114412 ICC/IF Image
FMO1 antibody [N2C1], Internal detects FMO1 protein at Cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in -20ºC 100% MeOH for 5 min.
Green: FMO1 protein stained by FMO1 antibody [N2C1], Internal (GTX114412) diluted at 1:500.
Blue: Hoechst 33343 staining.
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文献引用
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产品评论
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![Anti-FMO1 antibody [N2C1], Internal used in Western Blot (WB). GTX114412](/upload/website/prouct_img/small/GTX114412/GTX114412_WB_w_23060501_974.webp "Anti-FMO1 antibody [N2C1], Internal used in Western Blot (WB). GTX114412")
![Anti-FMO1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114412](/upload/website/prouct_img/small/GTX114412/GTX114412_40184_IFA_w_23060501_297.webp "Anti-FMO1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114412")
![Anti-FMO1 antibody [N2C1], Internal used in Western Blot (WB). GTX114412](/upload/website/prouct_img/normal/GTX114412/GTX114412_WB_w_23060501_974.webp "Anti-FMO1 antibody [N2C1], Internal used in Western Blot (WB). GTX114412")
![Anti-FMO1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114412](/upload/website/prouct_img/normal/GTX114412/GTX114412_40184_IFA_w_23060501_297.webp "Anti-FMO1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114412")
