产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
A549 , H1299 , HCT116 , FOXI1-transfected 293T , HepG2 nuclear extract
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human FOXI1. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_10622904
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
forkhead box I1 , FKH10 , FKHL10 , FREAC-6 , FREAC6 , HFH-3 , HFH3
功能与背景
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it is possible that this gene plays an important role in the development of the cochlea and vestibulum, as well as embryogenesis. Mutations in this gene may be associated with the common cavity phenotype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
数据库
研究领域
產品图片
|
GTX114408 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Huh7 xenograft, using FOXI1(GTX114408) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX114408 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FOXI1(GTX114408) antibody at 1:200 dilution.
|
|
GTX114408 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] (GTX114408) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX114408 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] (GTX114408) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX114408 WB Image
HepG2 whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] (GTX114408) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX114408 IHC-P Image
FOXI1 antibody [N2C3] detects FOXI1 protein at cytoplasm and nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. FOXI1 antibody [N2C3] (GTX114408) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX114408 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] (GTX114408) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
文献引用
目前没有使用 FOXI1 antibody [N2C3] (GTX114408) 的文献被收录。快来回报我们第一篇参考文献吧!
产品评论
目前没有使用 FOXI1 antibody [N2C3] (GTX114408) 的产品评论被收录。快发表第一篇产品评论吧!