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FOXO3A抗体 [C3], C-term

KO/KD Orthogonal
Anti-FOXO3A antibody [C3], C-term used in Western Blot (WB). GTX100277
Anti-FOXO3A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100277
Anti-FOXO3A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100277
Anti-FOXO3A antibody [C3], C-term used in Western Blot (WB). GTX100277
Anti-FOXO3A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100277
Anti-FOXO3A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100277
Anti-FOXO3A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100277
Anti-FOXO3A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100277
Anti-FOXO3A antibody [C3], C-term used in Immunoprecipitation (IP). GTX100277
Anti-FOXO3A antibody [C3], C-term used in Western Blot (WB). GTX100277
Anti-FOXO3A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100277
Anti-FOXO3A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100277
Anti-FOXO3A antibody [C3], C-term used in Western Blot (WB). GTX100277

目录号 GTX100277

文献引用 11

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human, Mouse, Rat, Squirrel, Xenopus
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

71 kDa. ( Note )

阳性对照

Mouse brain , C2C12

预测种属反应

Sheep, Bovine, Pig, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXO3A. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1240835

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

forkhead box O3 , AF6q21 , FKHRL1 , FKHRL1P2 , FOXO2 , FOXO3A

细胞定位

Cytoplasm , cytosol , Nucleus

功能与背景

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-FOXO3A antibody [C3], C-term used in Western Blot (WB). GTX100277

GTX100277 WB Image

KO/KD

Non-transfected (–) and transfected (+) C2C12 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FOXO3A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100277

GTX100277 IHC-P Image

FOXO3A antibody [C3], C-term detects FOXO3A protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
FOXO3A stained by FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FOXO3A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100277

GTX100277 IHC-P Image

FOXO3A antibody [C3], C-term detects FOXO3A protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
FOXO3A stained by FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FOXO3A antibody [C3], C-term used in Western Blot (WB). GTX100277

GTX100277 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX100277 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FOXO3A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100277

GTX100277 ICC/IF Image

FOXO3A antibody [C3], C-term detects FOXO3A protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FOXO3A stained by FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-FOXO3A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100277

GTX100277 IHC-P Image

FOXO3A antibody [C3], C-term detects FOXO3A protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
FOXO3A stained by FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FOXO3A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100277

GTX100277 IHC-P Image

FOXO3A antibody [C3], C-term detects FOXO3A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
FOXO3A stained by FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FOXO3A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100277

GTX100277 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Huh-7 xenograft, using FOXO3A(GTX100277) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FOXO3A antibody [C3], C-term used in Immunoprecipitation (IP). GTX100277

GTX100277 IP Image

FOXO3A antibody immunoprecipitates FOXO3A protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of FOXO3A protein by 2 μg of FOXO3A antibody (GTX101150) 5% SDS-PAGE The immunoprecipitated FOXO3A protein was detected by FOXO3A antibody (GTX101150) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-FOXO3A antibody [C3], C-term used in Western Blot (WB). GTX100277

GTX100277 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FOXO3A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100277

GTX100277 IHC-P Image

FOXO3A antibody [C3], C-term detects FOXO3A protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
FOXO3A stained by FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FOXO3A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100277

GTX100277 IHC-P Image

FOXO3A antibody [C3], C-term detects FOXO3A protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
FOXO3A stained by FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FOXO3A antibody [C3], C-term used in Western Blot (WB). GTX100277

GTX100277 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

文献引用

Application Reference

E Fletcher et al. Transl Res 2023; S1931-5244 (23) : 00088-9 Diet-induced obesity augments ischemic myopathy and functional decline in a murine model of peripheral artery disease
实验应用 : WB
Li CJ et al. J Hepatocell Carcinoma 2023; 10 : 123-138
实验应用 : WB
种属反应 : Human
Maiese A et al. Int J Mol Sci 2023; 24 (2) FOXO3 Depletion as a Marker of Compression-Induced Apoptosis in the Ligature Mark: An Immunohistochemical Study.
Maiese A et al. Int J Mol Sci 2023; 24 (3) The Expression of FOXO3a as a Forensic Diagnostic Tool in Cases of Traumatic Brain Injury: An Immunohistochemical Study.
实验应用 : IHC-P
种属反应 : Human
Li J et al. Ecotoxicol Environ Saf 2021; 228 : 112918 Effective-component compatibility of Bufei Yishen formula protects COPD rats against PM2.5-induced oxidative stress via miR-155/FOXO3a pathway.
实验应用 : WB
种属反应 : Rat
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Application Reference
E Fletcher et al. Transl Res 2023; S1931-5244 (23):00088-9 Diet-induced obesity augments ischemic myopathy and functional decline in a murine model of peripheral artery disease
实验应用 : WB
Li CJ et al. J Hepatocell Carcinoma 2023; 10:123-138
实验应用 : WB
种属反应 : Human
Maiese A et al. Int J Mol Sci 2023; 24 (2) FOXO3 Depletion as a Marker of Compression-Induced Apoptosis in the Ligature Mark: An Immunohistochemical Study.
Maiese A et al. Int J Mol Sci 2023; 24 (3) The Expression of FOXO3a as a Forensic Diagnostic Tool in Cases of Traumatic Brain Injury: An Immunohistochemical Study.
实验应用 : IHC-P
种属反应 : Human
Li J et al. Ecotoxicol Environ Saf 2021; 228:112918 Effective-component compatibility of Bufei Yishen formula protects COPD rats against PM2.5-induced oxidative stress via miR-155/FOXO3a pathway.
实验应用 : WB
种属反应 : Rat
Guo A et al. J Cell Mol Med 2021; 25 (7):3585-3600 FGF19 protects skeletal muscle against obesity-induced muscle atrophy, metabolic derangement and abnormal irisin levels via the AMPK/SIRT-1/PGC-α pathway.
实验应用 : WB
种属反应 : Mouse
Luu BE et al. Cell Stress Chaperones 2020; (Epub) The regulation of Akt and FoxO transcription factors during dehydration in the African clawed frog (Xenopus laevis).
实验应用 : WB
种属反应 : Xenopus
Chang CC et al. CNS Neurosci Ther 2017; (Epub) aβ exacerbates α-synuclein-induced neurotoxicity through impaired * signaling in α-synuclein-overexpressed human sk-n-mc neuronal cells.
实验应用 : WB
种属反应 : Human
Zhang Y et al. PeerJ 2016; 4:e2317 Transcriptional activation of muscle atrophy promotes cardiac muscle remodeling during mammalian hibernation.
实验应用 : WB
种属反应 : Squirrel
Yang WB et al. Oncotarget 2014; 5 (3):740-53 Sp1-mediated microRNA-182 expression regulates lung cancer progression.
实验应用 : WB
种属反应 : Human
Tseng HL et al. Toxicol Appl Pharmacol 2012; 264 (1):104-13 Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway.
实验应用 : WB
种属反应 : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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