产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
human F10-transfected 293T , GL261 , C8D30
预测种属反应
Rabbit, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.05 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Factor X. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1950240
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
coagulation factor X , FX , FXA
细胞定位
Secreted
功能与背景
This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. [provided by RefSeq]
数据库
研究领域
產品图片
|
GTX110300 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using Factor X(GTX110300) antibody at 1:200 dilution.
|
|
|
GTX110300 WB Image
Factor X antibody detects F10 protein by western blot analysis.
A. 30 μg GL261 whole lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
10% SDS-PAGE
Factor X antibody (GTX110300) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX110300 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Factor X(GTX110300) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
|
GTX110300 WB Image
Factor X antibody detects F10 protein by western blot analysis.
A. 30μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human F10-transfected 293T cells
10% SDS-PAGE
Factor X antibody (GTX110300) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX110300 WB Image
HepG2 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Factor X antibody (GTX110300) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
|
|
GTX110300 WB Image
The data was published in the journal Oncotarget in 2016.PMID: 27248165
|
|
|
GTX110300 WB Image
The data was published in the journal Oncotarget in 2016.PMID: 27248165
|
文献引用
产品评论
目前没有使用 Factor X antibody (GTX110300) 的产品评论被收录。快发表第一篇产品评论吧！
