产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
293T , A431 , HeLa , HepG2
预测种属反应
Bovine, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the N-terminus region of human Fatty Acid Synthase. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_2888153
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
fatty acid synthase , FAS , OA-519 , SDR27X1
细胞定位
Cytoplasm , Melanosome
功能与背景
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-Fatty Acid Synthase antibody [GT556] used in Western Blot (WB). GTX629762](/templates/default/js/lightbox/images/loading.gif "Anti-Fatty Acid Synthase antibody [GT556] used in Western Blot (WB). GTX629762")
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GTX629762 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [GT556] (GTX629762) diluted at 1:500.
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GTX629762 ICC/IF Image
Fatty Acid Synthase antibody [GT556] detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Fatty Acid Synthase stained by Fatty Acid Synthase antibody [GT556] (GTX629762) diluted at 1:100.Blue: Hoechst 33342 staining.
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GTX629762 IHC-P Image
Fatty Acid Synthase antibody [GT556] detects Fatty Acid Synthase protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Fatty Acid Synthase antibody [GT556] (GTX629762) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX629762 WB Image
Fatty Acid Synthase antibody [GT556] detects Fatty Acid Synthase protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
Fatty Acid Synthase antibody [GT556] (GTX629762) dilution: 1:1000
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GTX629762 IHC-P Image
Fatty Acid Synthase antibody [GT556] detects Fatty Acid Synthase protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Fatty Acid Synthase antibody [GT556] (GTX629762) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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产品评论
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![Anti-Fatty Acid Synthase antibody [GT556] used in Western Blot (WB). GTX629762](/upload/website/prouct_img/small/GTX629762/GTX629762_41435_20160616_WB_shRNA_watermark_w_23061202_832.webp "Anti-Fatty Acid Synthase antibody [GT556] used in Western Blot (WB). GTX629762")
![Anti-Fatty Acid Synthase antibody [GT556] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629762](/upload/website/prouct_img/small/GTX629762/GTX629762_41435_20180801_ICC_IF_w_23061202_366.webp "Anti-Fatty Acid Synthase antibody [GT556] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629762")
![Anti-Fatty Acid Synthase antibody [GT556] used in IHC (Paraffin sections) (IHC-P). GTX629762](/upload/website/prouct_img/small/GTX629762/GTX629762_41435_20160706_IHC-P_R_w_23061202_934.webp "Anti-Fatty Acid Synthase antibody [GT556] used in IHC (Paraffin sections) (IHC-P). GTX629762")
![Anti-Fatty Acid Synthase antibody [GT556] used in Western Blot (WB). GTX629762](/upload/website/prouct_img/small/GTX629762/GTX629762_41435_WB_w_23061202_853.webp "Anti-Fatty Acid Synthase antibody [GT556] used in Western Blot (WB). GTX629762")
![Anti-Fatty Acid Synthase antibody [GT556] used in IHC (Paraffin sections) (IHC-P). GTX629762](/upload/website/prouct_img/small/GTX629762/GTX629762_41435_20160706_IHC-P_M_w_23061202_820.webp "Anti-Fatty Acid Synthase antibody [GT556] used in IHC (Paraffin sections) (IHC-P). GTX629762")
![Anti-Fatty Acid Synthase antibody [GT556] used in Western Blot (WB). GTX629762](/upload/website/prouct_img/normal/GTX629762/GTX629762_41435_20160616_WB_shRNA_watermark_w_23061202_832.webp "Anti-Fatty Acid Synthase antibody [GT556] used in Western Blot (WB). GTX629762")
![Anti-Fatty Acid Synthase antibody [GT556] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629762](/upload/website/prouct_img/normal/GTX629762/GTX629762_41435_20180801_ICC_IF_w_23061202_366.webp "Anti-Fatty Acid Synthase antibody [GT556] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629762")
![Anti-Fatty Acid Synthase antibody [GT556] used in IHC (Paraffin sections) (IHC-P). GTX629762](/upload/website/prouct_img/normal/GTX629762/GTX629762_41435_20160706_IHC-P_R_w_23061202_934.webp "Anti-Fatty Acid Synthase antibody [GT556] used in IHC (Paraffin sections) (IHC-P). GTX629762")
![Anti-Fatty Acid Synthase antibody [GT556] used in Western Blot (WB). GTX629762](/upload/website/prouct_img/normal/GTX629762/GTX629762_41435_WB_w_23061202_853.webp "Anti-Fatty Acid Synthase antibody [GT556] used in Western Blot (WB). GTX629762")
![Anti-Fatty Acid Synthase antibody [GT556] used in IHC (Paraffin sections) (IHC-P). GTX629762](/upload/website/prouct_img/normal/GTX629762/GTX629762_41435_20160706_IHC-P_M_w_23061202_820.webp "Anti-Fatty Acid Synthase antibody [GT556] used in IHC (Paraffin sections) (IHC-P). GTX629762")
