产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
Assay dependent |
Assay dependent |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
2 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragmemt of human Fatty Acid Synthase
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
fatty acid synthase , FAS , OA-519 , SDR27X1
细胞定位
Cytoplasm,Melanosome
功能与背景
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX638139 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [HL2161] (GTX638139) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638139 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membranes were blotted with Fatty Acid Synthase antibody [HL2161] (GTX638139) diluted at 1:1000 and competitor's antibody (#Highly competitor antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX638139 ICC/IF Image
Fatty Acid Synthase antibody [HL2161] detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Fatty Acid Synthase stained by Fatty Acid Synthase antibody [HL2161] (GTX638139) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX638139 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [HL2161] (GTX638139) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638139 IHC-P Image
Fatty Acid Synthase antibody [HL2161] detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Fatty Acid Synthase stained by Fatty Acid Synthase antibody [HL2161] (GTX638139) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638139 WB Image
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [HL2161] (GTX638139) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638139 IHC-P Image
Fatty Acid Synthase antibody [HL2161] detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat liver.Fatty Acid Synthase stained by Fatty Acid Synthase antibody [HL2161] (GTX638139) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638139 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [HL2161] (GTX638139) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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