GeneTex中国官方网站
  • China

Fatty Acid Synthase抗体 [N1], N-term

KO/KD Orthogonal
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Immunoprecipitation (IP). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

目录号 GTX109833

文献引用 14
6

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, IP

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Fatty Acid Synthase 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

273 kDa. ( Note )

阳性对照

Mouse brain , Molt-4 , PC-12 , Rat2 , 293T

预测种属反应

Bovine, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human Fatty Acid Synthase. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950258

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

fatty acid synthase , FAS , OA-519 , SDR27X1

细胞定位

Cytoplasm , Melanosome

功能与背景

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

GTX109833 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

GTX109833 WB Image

Sample (30 μg of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX109833 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833

GTX109833 IHC-P Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833

GTX109833 IHC-P Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833

GTX109833 IHC-P Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse white adipocyte.
Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Fatty Acid Synthase antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109833

GTX109833 ICC/IF Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fatty Acid Synthase protein stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

GTX109833 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833

GTX109833 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian carainoma, using Fatty Acid Synthase(GTX109833) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

GTX109833 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fatty Acid Synthase antibody [N1], N-term used in Immunoprecipitation (IP). GTX109833

GTX109833 IP Image

FASN antibody immunoprecipitates FASN protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of FASN protein expressing HeLa cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FASN by 2.5 μg of FASN antibody (GTX109833) 5% SDS-PAGE The immunoprecipitated FASN protein was detected by FASN antibody (GTX109833) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833

GTX109833 IHC-P Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

GTX109833 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

GTX109833 WB Image

Orthogonal

The data was published in the journal Biomolecules in 2019. PMID: 31614639

文献引用

Application Reference

Wang SA et al. Mol Oncol 2023; NCI677397 targeting USP24-mediated induction of lipid peroxidation induces ferroptosis in drug-resistant cancer cells
实验应用 : WB
Giuliani A et al. Antioxidants (Basel) 2023; 12 (11) Senescent Endothelial Cells Sustain Their Senescence-Associated Secretory Phenotype (SASP) through Enhanced Fatty Acid Oxidation.
Tsao CH et al. Front Med (Lausanne) 2022; 9 : 1008200 Dual-tracer positron emission tomography/computed tomography as an imaging probe of de novo lipogenesis in preclinical models of hepatocellular carcinoma.
实验应用 : WB
种属反应 : Human
Ceja-Galicia ZA et al. Antioxidants (Basel) 2022; 11 (11) Therapeutic Effect of Curcumin on 5/6Nx Hypertriglyceridemia: Association with the Improvement of Renal Mitochondrial β-Oxidation and Lipid Metabolism in Kidney and Liver.
Lu Y et al. nanotoday 2022; 43 : 101416 Reactivation of dysfunctional dendritic cells by a stress-relieving nanosystem resets anti-tumor immune landscape.
显示更多 +
我要回报文献

产品评论

Fatty Acid Synthase antibody [N1], N-term 目录号 GTX109833

Rating
( Average 5 based on 6 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 6 users reviews)
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Date : Anonymous submitted on 18-Apr-2023
Score :
Application Tested : WB
Sample Species : Rat
Sample : adipose tissue
Amount used : 50μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Date : Anonymous submitted on 06-Sep-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 30 μg
Blocking : 5% milk in TBST, RT°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Secondary Antibody : GTX300119, 1:1000, RT°C, 1Hr
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Amount used : 35
Blocking : 5% milk in TBST buffer, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, > 12Hr
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Amount used : 35
Blocking : 5% milk in TBST buffer, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, > 12Hr
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Date : Anonymous submitted on 15-Jul-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 40 μg
Blocking : 5% BSA in TBST, 4°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Date : Anonymous submitted on 04-Aug-2016
Score :
Application Tested : WB
Sample Species : Rat
Sample : liver tissue
Amount used : 40μg
Blocking : 5% skim milk, 20°C, 1Hr
Primary Antibody : 1:6000, 4°C, 16Hr
显示更多 +
我要评论产品
Application Reference
Wang SA et al. Mol Oncol 2023; NCI677397 targeting USP24-mediated induction of lipid peroxidation induces ferroptosis in drug-resistant cancer cells
实验应用 : WB
Giuliani A et al. Antioxidants (Basel) 2023; 12 (11) Senescent Endothelial Cells Sustain Their Senescence-Associated Secretory Phenotype (SASP) through Enhanced Fatty Acid Oxidation.
Tsao CH et al. Front Med (Lausanne) 2022; 9:1008200 Dual-tracer positron emission tomography/computed tomography as an imaging probe of de novo lipogenesis in preclinical models of hepatocellular carcinoma.
实验应用 : WB
种属反应 : Human
Ceja-Galicia ZA et al. Antioxidants (Basel) 2022; 11 (11) Therapeutic Effect of Curcumin on 5/6Nx Hypertriglyceridemia: Association with the Improvement of Renal Mitochondrial β-Oxidation and Lipid Metabolism in Kidney and Liver.
Lu Y et al. nanotoday 2022; 43:101416 Reactivation of dysfunctional dendritic cells by a stress-relieving nanosystem resets anti-tumor immune landscape.
Park S et al. Exp Mol Med 2021; 53 (7):1159-1169 Loss of Acot12 contributes to NAFLD independent of lipolysis of adipose tissue.
实验应用 : IHC-Fr
种属反应 : Mouse
Raineri A et al. Molecules. 2021; 26 (16):5027 3,5-Dicaffeoylquinic Acid Lowers 3T3-L1 Mitotic Clonal Expansion and Adipocyte Differentiation by Enhancing Heme Oxygenase-1 Expression.
实验应用 : WB
种属反应 : Mouse
Tsai YT et al. Nutrients 2020; 12 (3) Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice.
Garc?a-Arroyo FE et al. Biomolecules 2019; 9 (10) Allopurinol Prevents the Lipogenic Response Induced by an Acute Oral Fructose Challenge in Short-Term Fructose Fed Rats.
You BJ et al. Sci Rep 2019; 9 (1):3306 Fenofibrate induces human hepatoma Hep3B cells apoptosis and necroptosis through inhibition of thioesterase domain of fatty acid synthase.
实验应用 : WB
种属反应 : Human
Ezzeddini R et al. Life Sci 2019; 224:169-176 Clinical importance of FASN in relation to HIF-1α and SREBP-1c in gastric adenocarcinoma.
实验应用 : WB
种属反应 : Human
Shimada M et al. Nutr Res 2017; 47:21-27 Dietary supplementation with myo-inositol reduces hepatic triglyceride accumulation and expression of both fructolytic and lipogenic genes in rats fed a high-fructose diet.
实验应用 : WB
种属反应 : Rat
Gui Y et al. Cardiovasc Res 2016; A novel small molecule liver X receptor transcriptional regulator, nagilactone B, suppresses atherosclerosis in apoE-deficient mice.
实验应用 : WB
种属反应 : Mouse
Jhuang HJ et al. Oncotarget 2015; 6 (10):7788-803 Gluconeogenesis, lipogenesis, and HBV replication are commonly regulated by PGC-1α-dependent pathway.
实验应用 : WB
种属反应 : Human
SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com