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Fibrillarin抗体 [38F3]

Anti-Fibrillarin antibody [38F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24566
Anti-Fibrillarin antibody [38F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24566
Anti-Fibrillarin antibody [38F3] used in Immunohistochemistry (IHC). GTX24566
Anti-Fibrillarin antibody [38F3] used in Western Blot (WB). GTX24566

目录号 GTX24566

文献引用 4

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 38F3

同种型

 IgG1

实验应用

 WB, ICC/IF, IHC-P, IHC

种属反应

 Human, Mouse, Rat, Yeast, Drosophila, Caenorhabditis elegans, Schizosaccharomyces pombe, Plant
产品包装
250 μl (¥4000)
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产品应用

应用说明

IF (ICC): 1/1000 - 1/5000 (yeast cells). For IF of mammalian cells, 1/500. WB: 1/2000 (cell lysates) - 1/10000 (nuclear fractions)(ECL). Detects a band of approximately 34 kDa. For other (non-ECL) western detection methods, 1/1000 - 1/5000. To detect mammalian fibrillarin on western blots by ECL, 1/500. Optimal dilutions/concentrations should be determined by the end user.

预期分子量

34 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

Tissue culture supernatant

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

免疫原种属

Human

免疫原

Yeast nuclear preparation (S. cerevisiae).Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae).

纯化方式

Unpurified

偶联

Unconjugated

RRID

AB_367009

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

fibrillarin , FIB , FLRN , Nop1 , RNU3IP1

细胞定位

Nucleolus

功能与背景

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-Fibrillarin antibody [38F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24566

GTX24566 ICC/IF Image

Human neuroblastoma line SH-SY5Y stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to neurofilament NF-H (red) and counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000

Anti-Fibrillarin antibody [38F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24566

GTX24566 ICC/IF Image

High magnification view of human Hek293 cell nuclei stained with mouse monoclonal to fibrillarin (green), counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). Cultures were processed using our standard fixat

Anti-Fibrillarin antibody [38F3] used in Immunohistochemistry (IHC). GTX24566

GTX24566 IHC Image

Rat neurons and glial stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to neurofilament NF-H (red). Cells were counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue).

Anti-Fibrillarin antibody [38F3] used in Western Blot (WB). GTX24566

GTX24566 WB Image

Blot of yeast protein extract stained with the GTX24566 anti-Gibrillarin anitbody, stained a single band at ~34kDa.

文献引用

Application Reference

Bennett CL et al. Heliyon 2020; 6 (6) : e04165 Tight expression regulation of senataxin, linked to motor neuron disease and ataxia, is required to avert cell-cycle block and nucleolus disassembly.
Xiangduo Kong et al. bioRxiv 2019; (Epub) The cohesin loader NIPBL interacts with pre-ribosomal RNA and treacle to regulate ribosomal RNA synthesis.
实验应用 : ICC/IF
种属反应 : Human
Behm M et al. J Cell Sci 2017; 130 (4) : 745-753 Accumulation of nuclear ADAR2 regulates adenosine-to-inosine RNA editing during neuronal development.
实验应用 : ICC/IF
种属反应 : Mouse
Zougman A et al. Nucleic Acids Res 2011; 39 (16) : 7103-13 Identification and characterization of a novel ubiquitous nucleolar protein 'NARR' encoded by a gene overlapping the rab34 oncogene.
实验应用 : ICC/IF
种属反应 : Human
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产品包装 报价 (¥)
¥ 4000
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