产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Rat2 , 293T , H1299 , NIH-3T3
产品说明
IP/MS validation was supported by references (PMID:30377378)
预测种属反应
Zebrafish, Bovine(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Fibrillarin. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1240813
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
fibrillarin , FIB , FLRN , Nop1 , RNU3IP1
细胞定位
Nucleus , nucleolus
功能与背景
This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX101807 WB Image
Sample (30 ug of whole cell lysate)
A:NIH-3T3 10% SDS PAGE
GTX101807 diluted at 1:1000
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GTX101807 IP Image
Fibrillarin antibody immunoprecipitates FBL protein in IP experiments.
IP samples: HeLa nuclear extract
A. 35 μg HeLa nuclear extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of FBL protein by 4 μg Fibrillarin antibody (GTX101807)
10 % SDS-PAGE
The immunoprecipitated FBL protein was detected by Fibrillarin antibody (GTX101807) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX101807 WB Image
Sample (30 ug of whole cell lysate)
A: 293T
B: H1299
12% SDS PAGE
GTX101807 diluted at 1:1000
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GTX101807 WB Image
Fibrillarin antibody detects FBL protein by Western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
12 % SDS-PAGE
Fibrillarin antibody (GTX101807) dilution: 1:1000
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GTX101807 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Fibrillarin(GTX101807) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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文献引用
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