产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
PC-12 , 293T , H1299 , Molt-4 , Neuro2A , A549 nuclear extract , Jurkat nuclear extract , SK-N-SH nuclear extract , IMR32 nuclear extract
预测种属反应
Bovine, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 10% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.36 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Fibrillarin. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2036928
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
fibrillarin , FIB , FLRN , Nop1 , RNU3IP1
细胞定位
Nucleus , nucleolus
功能与背景
This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX113684 IHC-P Image
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Fibrillarin antibody (GTX113684) at 1:300 dilution.
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GTX113684 WB Image
Sample (30 μg of whole cell lysate)
A: zebrafish eye
10% SDS PAGE
GTX113684 diluted at 1:1000
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GTX113684 IHC-P Image
Fibrillarin antibody detects Fibrillarin protein at nucleolus in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
Fibrillarin antibody (GTX113684) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113684 WB Image
Fibrillarin antibody detects Fibrillarin protein by western blot analysis.
whole cell lysate (30 μg) and nuclear extract (30 μg) from IMR32 cells
10 % SDS-PAGE
Fibrillarin antibody (GTX113684) dilution: 1:500
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GTX113684 WB Image
Fibrillarin antibody detects Fibrillarin protein by western blot analysis.
whole cell lysate (30 μg) and nuclear extract(30 μg) from A549 cells
10 % SDS-PAGE
Fibrillarin antibody (GTX113684) dilution: 1:500
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GTX113684 WB Image
Fibrillarin antibody detects Fibrillarin protein by western blot analysis.
whole cell lysate (30 μg) and nuclear extract (30 μg) from Jurkat cells
10 % SDS-PAGE
Fibrillarin antibody (GTX113684) dilution: 1:500
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GTX113684 WB Image
Fibrillarin antibody detects Fibrillarin protein by western blot analysis.
whole cell lysate (30 μg) and nuclear extract (30 μg) from SK-N-SH cells
10 % SDS-PAGE
Fibrillarin antibody (GTX113684) dilution: 1:500
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GTX113684 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using Fibrillarin (GTX113684) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113684 WB Image
Fibrillarin antibody detects FBL protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12 % SDS-PAGE
Fibrillarin antibody (GTX113684) dilution: 1:500
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GTX113684 WB Image
Sample (30 ug of whole cell lysate)
A: 293T
B: H1299
C: Molt-4 (GTX27912)
12% SDS PAGE
GTX113684 diluted at 1:1000
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GTX113684 WB Image
Fibrillarin antibody detects FBL protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
12 % SDS-PAGE
Fibrillarin antibody (GTX113684) dilution: 1:1000
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GTX113684 ICC/IF Image
Fibrillarin antibody detects Fibrillarin protein at nucleus, nucleolus and cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Fibrillarin stained by Fibrillarin antibody (GTX113684) diluted at 1:2000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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文献引用
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产品评论
Fibrillarin antibody
目录号 GTX113684
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Rating
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( Average 4 based on 1 users reviews)
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实验应用
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Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 05-Oct-2019
Application Tested :
WB
Sample Species :
Hu
Sample :
Huh7 cells
Amount used :
5 μg
Blocking :
2.5% non-fat milk, 25°C, 0.5Hr
Primary Antibody :
1:2000, 25°C, 4Hr
