产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:100000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Bovine, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Fibrinogen gamma. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2910083
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
fibrinogen gamma chain
细胞定位
Secreted
功能与背景
The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]
数据库
研究领域
產品图片
![Anti-Fibrinogen gamma antibody [HL1395] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636835](/templates/default/js/lightbox/images/loading.gif "Anti-Fibrinogen gamma antibody [HL1395] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636835")
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GTX636835 ICC/IF Image
Fibrinogen gamma antibody [HL1395] detects Fibrinogen gamma protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fibrinogen gamma stained by Fibrinogen gamma antibody [HL1395] (GTX636835) diluted at 1:50.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.
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GTX636835 IHC-P Image
Fibrinogen gamma antibody [HL1395] detects secreted Fibrinogen gamma protein by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Fibrinogen gamma stained by Fibrinogen gamma antibody [HL1395] (GTX636835) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636835 WB Image
Human plasma (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody [HL1395] (GTX636835) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636835 WB Image
Human plasma (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody [HL1395] (GTX636835) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636835 WB Image
Mouse plasma and rat plasma (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody [HL1395] (GTX636835) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-Fibrinogen gamma antibody [HL1395] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636835](/upload/website/prouct_img/small/GTX636835/GTX636835_44662_20220624_ICC_IF_22062919_851.webp "Anti-Fibrinogen gamma antibody [HL1395] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636835")
![Anti-Fibrinogen gamma antibody [HL1395] used in IHC (Paraffin sections) (IHC-P). GTX636835](/upload/website/prouct_img/small/GTX636835/GTX636835_44662_20220603_IHC-P_22062919_852.webp "Anti-Fibrinogen gamma antibody [HL1395] used in IHC (Paraffin sections) (IHC-P). GTX636835")
![Anti-Fibrinogen gamma antibody [HL1395] used in Western Blot (WB). GTX636835](/upload/website/prouct_img/small/GTX636835/GTX636835_44699_20220624_WB_plasma_22082402_742.webp "Anti-Fibrinogen gamma antibody [HL1395] used in Western Blot (WB). GTX636835")
![Anti-Fibrinogen gamma antibody [HL1395] used in Western Blot (WB). GTX636835](/upload/website/prouct_img/small/GTX636835/GTX636835_44662_20220429_WB_plasma_22082402_739.webp "Anti-Fibrinogen gamma antibody [HL1395] used in Western Blot (WB). GTX636835")
![Anti-Fibrinogen gamma antibody [HL1395] used in Western Blot (WB). GTX636835](/upload/website/prouct_img/small/GTX636835/GTX636835_44662_20220429_WB_M_R_22082402_750.webp "Anti-Fibrinogen gamma antibody [HL1395] used in Western Blot (WB). GTX636835")
![Anti-Fibrinogen gamma antibody [HL1395] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636835](/upload/website/prouct_img/normal/GTX636835/GTX636835_44662_20220624_ICC_IF_22062919_851.webp "Anti-Fibrinogen gamma antibody [HL1395] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636835")
![Anti-Fibrinogen gamma antibody [HL1395] used in IHC (Paraffin sections) (IHC-P). GTX636835](/upload/website/prouct_img/normal/GTX636835/GTX636835_44662_20220603_IHC-P_22062919_852.webp "Anti-Fibrinogen gamma antibody [HL1395] used in IHC (Paraffin sections) (IHC-P). GTX636835")
![Anti-Fibrinogen gamma antibody [HL1395] used in Western Blot (WB). GTX636835](/upload/website/prouct_img/normal/GTX636835/GTX636835_44699_20220624_WB_plasma_22082402_742.webp "Anti-Fibrinogen gamma antibody [HL1395] used in Western Blot (WB). GTX636835")
![Anti-Fibrinogen gamma antibody [HL1395] used in Western Blot (WB). GTX636835](/upload/website/prouct_img/normal/GTX636835/GTX636835_44662_20220429_WB_plasma_22082402_739.webp "Anti-Fibrinogen gamma antibody [HL1395] used in Western Blot (WB). GTX636835")
![Anti-Fibrinogen gamma antibody [HL1395] used in Western Blot (WB). GTX636835](/upload/website/prouct_img/normal/GTX636835/GTX636835_44662_20220429_WB_M_R_22082402_750.webp "Anti-Fibrinogen gamma antibody [HL1395] used in Western Blot (WB). GTX636835")
