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Fibrinogen gamma抗体

Anti-Fibrinogen gamma antibody used in Immunoprecipitation (IP). GTX108640
Anti-Fibrinogen gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108640
Anti-Fibrinogen gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108640
Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX108640
Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX108640
Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX108640
Anti-Fibrinogen gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX108640

目录号 GTX108640

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IP

种属反应

Human, Mouse, Rat, Bovine
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

52 kDa. ( Note )

阳性对照

rat plasma

预测种属反应

Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.77 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Fibrinogen gamma. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950280

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

fibrinogen gamma chain

细胞定位

Secreted

功能与背景

The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Fibrinogen gamma antibody used in Immunoprecipitation (IP). GTX108640

GTX108640 IP Image

Immunoprecipitation of Fibrinogen gamma protein from HepG2 whole cell extracts using 5 μg of Fibrinogen gamma antibody (GTX108640).
Western blot analysis was performed using Fibrinogen gamma antibody (GTX108640).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Fibrinogen gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108640

GTX108640 ICC/IF Image

Fibrinogen gamma antibody detects Fibrinogen gamma protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fibrinogen gamma protein stained by Fibrinogen gamma antibody (GTX108640) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

Anti-Fibrinogen gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108640

GTX108640 ICC/IF Image

Fibrinogen gamma antibody detects Fibrinogen gamma protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fibrinogen gamma stained by Fibrinogen gamma antibody (GTX108640) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX108640

GTX108640 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody (GTX108640) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX108640

GTX108640 WB Image

Mouse plasma and rat plasma (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody (GTX108640) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX108640

GTX108640 WB Image

Human plasma and HepG2 conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody (GTX108640) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fibrinogen gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX108640

GTX108640 IHC-P Image

Fibrinogen gamma antibody detects FGG protein at cytosol on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
Fibrinogen gamma antibody (GTX108640) dilution: 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

产品评论

Fibrinogen gamma antibody 目录号 GTX108640

Rating ( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 15-May-2018
Score :
Application Tested : WB
Sample Species : Bov
Blocking : 2% skim milk, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 18Hr
SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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