产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
rat liver , mouse liver , 293T , A431 , HeLa , HepG2
预测种属反应
Zebrafish, Sheep, Bovine, Chicken, Pig, Chimpanzee(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Fumarate hydratase. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_2888187
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
fumarate hydratase , FMRD , HLRCC , LRCC , MCL , MCUL1
细胞定位
Mitochondrion , Cytoplasm
功能与背景
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-Fumarate hydratase antibody [GT7612] used in Western Blot (WB). GTX630146](/templates/default/js/lightbox/images/loading.gif "Anti-Fumarate hydratase antibody [GT7612] used in Western Blot (WB). GTX630146")
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GTX630146 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [GT7612] (GTX630146) diluted at 1:500.
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GTX630146 WB Image
Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein by Western blot analysis.
A. 50 μg rat liver lysate/extract
10 % SDS-PAGE
Fumarate hydratase antibody [GT7612] (GTX630146) dilution: 1:1000
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GTX630146 ICC/IF Image
Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Fumarate hydratase protein stained by Fumarate hydratase antibody [GT7612] (GTX630146) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX630146 WB Image
Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
10 % SDS-PAGE
Fumarate hydratase antibody [GT7612] (GTX630146) dilution: 1:1000
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GTX630146 WB Image
Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein by Western blot analysis.
A. 30 μg 293T
B. 30 μg A431
C. 30 μg HeLa
D. 30 μg HepG2
10 % SDS-PAGE
Fumarate hydratase antibody [GT7612] (GTX630146) dilution: 1:500
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文献引用
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产品评论
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![Anti-Fumarate hydratase antibody [GT7612] used in Western Blot (WB). GTX630146](/upload/website/prouct_img/small/GTX630146/GTX630146_41498_20160721_WB_shRNA_watermark_w_23061202_654.webp "Anti-Fumarate hydratase antibody [GT7612] used in Western Blot (WB). GTX630146")
![Anti-Fumarate hydratase antibody [GT7612] used in Western Blot (WB). GTX630146](/upload/website/prouct_img/small/GTX630146/GTX630146_41498_WB_R_liver_w_23061202_292.webp "Anti-Fumarate hydratase antibody [GT7612] used in Western Blot (WB). GTX630146")
![Anti-Fumarate hydratase antibody [GT7612] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630146](/upload/website/prouct_img/small/GTX630146/GTX630146_41498_20170719_IFA_w_23061202_312.webp "Anti-Fumarate hydratase antibody [GT7612] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630146")
![Anti-Fumarate hydratase antibody [GT7612] used in Western Blot (WB). GTX630146](/upload/website/prouct_img/small/GTX630146/GTX630146_41498_WB_M_liver_w_23061202_541.webp "Anti-Fumarate hydratase antibody [GT7612] used in Western Blot (WB). GTX630146")
![Anti-Fumarate hydratase antibody [GT7612] used in Western Blot (WB). GTX630146](/upload/website/prouct_img/small/GTX630146/GTX630146_41498_WB_w_23061202_279.webp "Anti-Fumarate hydratase antibody [GT7612] used in Western Blot (WB). GTX630146")
![Anti-Fumarate hydratase antibody [GT7612] used in Western Blot (WB). GTX630146](/upload/website/prouct_img/normal/GTX630146/GTX630146_41498_20160721_WB_shRNA_watermark_w_23061202_654.webp "Anti-Fumarate hydratase antibody [GT7612] used in Western Blot (WB). GTX630146")
![Anti-Fumarate hydratase antibody [GT7612] used in Western Blot (WB). GTX630146](/upload/website/prouct_img/normal/GTX630146/GTX630146_41498_WB_R_liver_w_23061202_292.webp "Anti-Fumarate hydratase antibody [GT7612] used in Western Blot (WB). GTX630146")
![Anti-Fumarate hydratase antibody [GT7612] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630146](/upload/website/prouct_img/normal/GTX630146/GTX630146_41498_20170719_IFA_w_23061202_312.webp "Anti-Fumarate hydratase antibody [GT7612] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630146")
![Anti-Fumarate hydratase antibody [GT7612] used in Western Blot (WB). GTX630146](/upload/website/prouct_img/normal/GTX630146/GTX630146_41498_WB_M_liver_w_23061202_541.webp "Anti-Fumarate hydratase antibody [GT7612] used in Western Blot (WB). GTX630146")
![Anti-Fumarate hydratase antibody [GT7612] used in Western Blot (WB). GTX630146](/upload/website/prouct_img/normal/GTX630146/GTX630146_41498_WB_w_23061202_279.webp "Anti-Fumarate hydratase antibody [GT7612] used in Western Blot (WB). GTX630146")
