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Fumarate hydratase抗体 [N2C2], Internal

KO/KD Orthogonal Overexpression
Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128
Anti-Fumarate hydratase antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110128
Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128
Anti-Fumarate hydratase antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110128
Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128
Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128

目录号 GTX110128

文献引用 4

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

55 kDa. ( Note )

阳性对照

mouse liver , 293T , A431 , rat liver

预测种属反应

Zebrafish, Sheep, Bovine, Chicken, Pig, Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Fumarate hydratase. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950282

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

fumarate hydratase , FMRD , HLRCC , LRCC , MCL , MCUL1

细胞定位

Mitochondrion , Cytoplasm

功能与背景

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128

GTX110128 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [N2C2], Internal (GTX110128) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fumarate hydratase antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110128

GTX110128 ICC/IF Image

Fumarate hydratase antibody [N2C2], Internal detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Fumarate hydratase protein stained by Fumarate hydratase antibody [N2C2], Internal (GTX110128) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128

GTX110128 WB Image

Fumarate hydratase antibody [N2C2], Internal detects FH protein by western blot analysis.
A. 50 μg mouse liver lysate/extract
10% SDS-PAGE
Fumarate hydratase antibody [N2C2], Internal (GTX110128) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fumarate hydratase antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110128

GTX110128 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using fumarate hydratase (GTX110128) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128

GTX110128 WB Image

Fumarate hydratase antibody [N2C2], Internal detects Fumarate hydratase protein by western blot analysis.
A.50 μg rat liver lysate/extract
10% SDS-PAGE
Fumarate hydratase antibody [N2C2], Internal (GTX110128) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128

GTX110128 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431 (GTX27909)
10% SDS PAGE
GTX110128 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Vadhan A et al. Oncol Lett 2023; 25 (1) : 42 Fumarate hydratase inhibits non-small cell lung cancer metastasis via inactivation of AMPK and upregulation of DAB2.
实验应用 : WB, IHC-P
种属反应 : Human
Lee SY et al. Oncol Rep 2021; 45 (4) Bcl2l10 induces metabolic alterations in ovarian cancer cells by regulating the TCA cycle enzymes SDHD and IDH1.
实验应用 : WB
种属反应 : Human
Tseng CW et al. Cancer Res 2018; (Epub) Transketolase regulates the metabolic switch to control breast cancer cell metastasis via the alpha-ketoglutarate signaling pathway.
实验应用 : WB
种属反应 : Human
Shanmugasundaram K et al. J Biol Chem 2014; The Oncometabolite Fumarate Promotes Pseudohypoxia Through Noncannonical Activation of NF-κB Signaling.
实验应用 : WB
种属反应 : Human
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Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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