产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
human GABARAPL1-transfected 293T cells
预测种属反应
Mouse, Rat, Rabbit, Bovine, Guinea pig, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Full length human GABARAPL1 Recombinant protein.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2885951
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
GABA type A receptor associated protein like 1 , APG8-LIKE , APG8L , ATG8 , ATG8B , ATG8L , GEC1
细胞定位
Cytoplasm , Membrane , Endoplasmic reticulum , Golgi apparatus
功能与背景
Increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor.
数据库
研究领域
產品图片
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GTX129277 ICC/IF Image
GABARAPL1 antibody detects GABARAPL1 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: GABARAPL1 protein stained by GABARAPL1 antibody (GTX129277) diluted at 1:1000. Red: Phalloidin, a F-actin marker.
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GTX129277 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with GABARAPL1 antibody (GTX129277) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX129277 WB Image
GABARAPL1 antibody detects GABARAPL1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of human GABARAPL1-transfected 293T cells 15 % SDS-PAGE GABARAPL1 antibody (GTX129277) dilution: 1:1000
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