Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GAD65 antibody [HL1187] detects GAD65 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD65 stained by GAD65 antibody [HL1187] (GTX636493) diluted at 1:500.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GAD65 antibody [HL1187] detects GAD65 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: GAD65 stained by GAD65 antibody [HL1187] (GTX636493) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GAD65 antibody [HL1187] detects GAD65 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat cerebellum.
GAD65 stained by GAD65 antibody [HL1187] (GTX636493) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAD65 antibody [HL1187] detects GAD65 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog cerebellum.
GAD65 stained by GAD65 antibody [HL1187] (GTX636493) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.