产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for GAD65 protein, and it does not cross-react with GAD67 protein.
预测种属反应
Bovine, Pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the C-terminus region of human GAD65. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2909995
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
glutamate decarboxylase 2 , GAD65
细胞定位
Cytoplasm, cytosol,Cytoplasmic vesicle,Golgi apparatus membrane
功能与背景
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]
数据库
研究领域
產品图片
![Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493](/templates/default/js/lightbox/images/loading.gif "Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493")
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GTX636493 IHC-P Image
GAD65 antibody [HL1187] detects GAD65 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog cerebellum.GAD65 stained by GAD65 antibody [HL1187] (GTX636493) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636493 IHC-P Image
GAD65 antibody [HL1187] detects GAD65 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat cerebellum.GAD65 stained by GAD65 antibody [HL1187] (GTX636493) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636493 WB Image
Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX636493 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX636493 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636493 WB Image
Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636493 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636493 IHC-P Image
GAD65 antibody [HL1187] detects GAD65 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: GAD65 stained by GAD65 antibody [HL1187] (GTX636493) diluted at 1:100.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636493 ICC/IF Image
GAD65 antibody [HL1187] detects GAD65 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GAD65 stained by GAD65 antibody [HL1187] (GTX636493) diluted at 1:500.Red: Tau, a Tau marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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文献引用
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产品评论
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![Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493](/upload/website/prouct_img/small/GTX636493/GTX636493_44522_20230203_IHC-P_Dog_23021401_837.webp "Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493")
![Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493](/upload/website/prouct_img/small/GTX636493/GTX636493_44522_20230203_IHC-P_Cat_23021401_592.webp "Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493")
![Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493](/upload/website/prouct_img/small/GTX636493/GTX636493_44522_20221216_WB_Z_22122018_598.webp "Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493")
![Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493](/upload/website/prouct_img/small/GTX636493/GTX636493_44522_20221223_WB_D_C_22122018_138.webp "Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493")
![Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493](/upload/website/prouct_img/small/GTX636493/GTX636493_44522_20211224_WB_M_R_w_23061202_796.webp "Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493")
![Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493](/upload/website/prouct_img/small/GTX636493/GTX636493_T-44445_20211029_WB_brain_w_23061202_876.webp "Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493")
![Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493](/upload/website/prouct_img/small/GTX636493/GTX636493_44522_20220429_WB_B_w_23061202_172.webp "Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493")
![Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493](/upload/website/prouct_img/small/GTX636493/GTX636493_44522_20220107_IHC-P_M_w_23061202_622.webp "Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493")
![Anti-GAD65 antibody [HL1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636493](/upload/website/prouct_img/small/GTX636493/GTX636493_44522_20220128_ICC_IF_w_23061202_830.webp "Anti-GAD65 antibody [HL1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636493")
![Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493](/upload/website/prouct_img/normal/GTX636493/GTX636493_44522_20230203_IHC-P_Dog_23021401_837.webp "Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493")
![Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493](/upload/website/prouct_img/normal/GTX636493/GTX636493_44522_20230203_IHC-P_Cat_23021401_592.webp "Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493")
![Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493](/upload/website/prouct_img/normal/GTX636493/GTX636493_44522_20221216_WB_Z_22122018_598.webp "Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493")
![Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493](/upload/website/prouct_img/normal/GTX636493/GTX636493_44522_20221223_WB_D_C_22122018_138.webp "Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493")
![Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493](/upload/website/prouct_img/normal/GTX636493/GTX636493_44522_20211224_WB_M_R_w_23061202_796.webp "Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493")
![Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493](/upload/website/prouct_img/normal/GTX636493/GTX636493_T-44445_20211029_WB_brain_w_23061202_876.webp "Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493")
![Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493](/upload/website/prouct_img/normal/GTX636493/GTX636493_44522_20220429_WB_B_w_23061202_172.webp "Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493")
![Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493](/upload/website/prouct_img/normal/GTX636493/GTX636493_44522_20220107_IHC-P_M_w_23061202_622.webp "Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493")
![Anti-GAD65 antibody [HL1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636493](/upload/website/prouct_img/normal/GTX636493/GTX636493_44522_20220128_ICC_IF_w_23061202_830.webp "Anti-GAD65 antibody [HL1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636493")
