产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
mouse brain , rat brain
预测种属反应
Human, Bovine, Dog, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.34 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Mouse
免疫原
Recombinant protein encompassing a sequence within the N-terminus region of mouse GAD65. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2886849
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
glutamic acid decarboxylase 2 , 6330404F12Rik , GAD(65) , GAD65 , Gad-2
细胞定位
Cytoplasm , cytosol , Cytoplasmic vesicle , Cell junction , synapse , presynaptic cell membrane; Lipid-anchor , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , cytosol , Cytoplasmic vesicle , Cell junction , synapse
功能与背景
Catalyzes the production of GABA.
数据库
研究领域
產品图片
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GTX133156 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody (GTX133156) diluted at 1:1000.
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GTX133156 IHC-P Image
GAD65 antibody detects GAD65 protein expression by immunohistochemical analysis.
Sample: Paraffin-Embedded adult mouse retina.
Green: GAD65 protein stained by GAD65 antibody (GTX133156) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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