产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| Assay dependent |
| 1:100-1:1000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for GAD67 protein, and it does not cross-react with GAD65 protein.
预测种属反应
Bovine, Chicken, Pig, Chimpanzee(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human GAD67. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2909958
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
glutamate decarboxylase 1 , CPSQ1 , GAD , SCP
功能与背景
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-GAD67 antibody [HL1095] used in IHC (Paraffin sections) (IHC-P). GTX636279](/templates/default/js/lightbox/images/loading.gif "Anti-GAD67 antibody [HL1095] used in IHC (Paraffin sections) (IHC-P). GTX636279")
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GTX636279 IHC-P Image
GAD67 antibody [HL1095] detects GAD67 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.GAD67 stained by GAD67 antibody [HL1095] (GTX636279) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636279 ICC/IF Image
GAD67 antibody [HL1095] detects GAD67 protein at cytoplasm by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GAD67 stained by GAD67 antibody [HL1095] (GTX636279) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636279 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1095] (GTX636279) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636279 ICC/IF Image
GAD67 antibody [HL1095] detects GAD67 protein by immunofluorescent analysis.Sample: DIV9 rat cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GAD67 stained by GAD67 antibody [HL1095] (GTX636279) diluted at 1:500.Red: Tau, a Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
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GTX636279 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1095] (GTX636279) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636279 WB Image
Human tissue extract (10 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1095] (GTX636279) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636279 IHC-P Image
GAD67 antibody [HL1095] detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat cerebellum.GAD67 stained by GAD67 antibody [HL1095] (GTX636279) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636279 IHC-P Image
GAD67 antibody [HL1095] detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog cerebellum.GAD67 stained by GAD67 antibody [HL1095] (GTX636279) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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![Anti-GAD67 antibody [HL1095] used in IHC (Paraffin sections) (IHC-P). GTX636279](/upload/website/prouct_img/small/GTX636279/GTX636279_44536_20221227_IHC-P_multiple_M_22122821_457.webp "Anti-GAD67 antibody [HL1095] used in IHC (Paraffin sections) (IHC-P). GTX636279")
![Anti-GAD67 antibody [HL1095] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636279](/upload/website/prouct_img/small/GTX636279/GTX636279_44536_20220311_ICC_IF_ICON00403_w_23061202_124.webp "Anti-GAD67 antibody [HL1095] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636279")
![Anti-GAD67 antibody [HL1095] used in Western Blot (WB). GTX636279](/upload/website/prouct_img/small/GTX636279/GTX636279_44536_20211231_WB_M_R_w_23061202_605.webp "Anti-GAD67 antibody [HL1095] used in Western Blot (WB). GTX636279")
![Anti-GAD67 antibody [HL1095] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636279](/upload/website/prouct_img/small/GTX636279/GTX636279_T-44347_20211112_ICC_IF_R_w_23061202_671.webp "Anti-GAD67 antibody [HL1095] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636279")
![Anti-GAD67 antibody [HL1095] used in Western Blot (WB). GTX636279](/upload/website/prouct_img/small/GTX636279/GTX636279_44536_20220429_WB_B_w_23061202_431.webp "Anti-GAD67 antibody [HL1095] used in Western Blot (WB). GTX636279")
![Anti-GAD67 antibody [HL1095] used in Western Blot (WB). GTX636279](/upload/website/prouct_img/small/GTX636279/GTX636279_44536_20220401_WB_brain_w_23061202_843.webp "Anti-GAD67 antibody [HL1095] used in Western Blot (WB). GTX636279")
![Anti-GAD67 antibody [HL1095] used in IHC (Paraffin sections) (IHC-P). GTX636279](/upload/website/prouct_img/small/GTX636279/GTX636279_44536_20230203_IHC-P_Cat_23021401_584.webp "Anti-GAD67 antibody [HL1095] used in IHC (Paraffin sections) (IHC-P). GTX636279")
![Anti-GAD67 antibody [HL1095] used in IHC (Paraffin sections) (IHC-P). GTX636279](/upload/website/prouct_img/small/GTX636279/GTX636279_44536_20230203_IHC-P_Dog_23021401_493.webp "Anti-GAD67 antibody [HL1095] used in IHC (Paraffin sections) (IHC-P). GTX636279")
![Anti-GAD67 antibody [HL1095] used in IHC (Paraffin sections) (IHC-P). GTX636279](/upload/website/prouct_img/normal/GTX636279/GTX636279_44536_20221227_IHC-P_multiple_M_22122821_457.webp "Anti-GAD67 antibody [HL1095] used in IHC (Paraffin sections) (IHC-P). GTX636279")
![Anti-GAD67 antibody [HL1095] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636279](/upload/website/prouct_img/normal/GTX636279/GTX636279_44536_20220311_ICC_IF_ICON00403_w_23061202_124.webp "Anti-GAD67 antibody [HL1095] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636279")
![Anti-GAD67 antibody [HL1095] used in Western Blot (WB). GTX636279](/upload/website/prouct_img/normal/GTX636279/GTX636279_44536_20211231_WB_M_R_w_23061202_605.webp "Anti-GAD67 antibody [HL1095] used in Western Blot (WB). GTX636279")
![Anti-GAD67 antibody [HL1095] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636279](/upload/website/prouct_img/normal/GTX636279/GTX636279_T-44347_20211112_ICC_IF_R_w_23061202_671.webp "Anti-GAD67 antibody [HL1095] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636279")
![Anti-GAD67 antibody [HL1095] used in Western Blot (WB). GTX636279](/upload/website/prouct_img/normal/GTX636279/GTX636279_44536_20220429_WB_B_w_23061202_431.webp "Anti-GAD67 antibody [HL1095] used in Western Blot (WB). GTX636279")
![Anti-GAD67 antibody [HL1095] used in Western Blot (WB). GTX636279](/upload/website/prouct_img/normal/GTX636279/GTX636279_44536_20220401_WB_brain_w_23061202_843.webp "Anti-GAD67 antibody [HL1095] used in Western Blot (WB). GTX636279")
![Anti-GAD67 antibody [HL1095] used in IHC (Paraffin sections) (IHC-P). GTX636279](/upload/website/prouct_img/normal/GTX636279/GTX636279_44536_20230203_IHC-P_Cat_23021401_584.webp "Anti-GAD67 antibody [HL1095] used in IHC (Paraffin sections) (IHC-P). GTX636279")
![Anti-GAD67 antibody [HL1095] used in IHC (Paraffin sections) (IHC-P). GTX636279](/upload/website/prouct_img/normal/GTX636279/GTX636279_44536_20230203_IHC-P_Dog_23021401_493.webp "Anti-GAD67 antibody [HL1095] used in IHC (Paraffin sections) (IHC-P). GTX636279")
