产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
H1299 , Neuro2A , C2C12 , PC-12 , Rat2
预测种属反应
Zebrafish, Bovine, Dog, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 10% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human GALNS. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1950332
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
galactosamine (N-acetyl)-6-sulfatase , GALNAC6S , GAS , GalN6S , MPS4A
细胞定位
Lysosome
功能与背景
This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder. [provided by RefSeq]
数据库
研究领域
產品图片
|
GTX110237 WB Image
GALNS antibody detects GALNS protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 7.5% SDS-PAGE GALNS antibody (GTX110237) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX110237 WB Image
Sample (30 μg of whole cell lysate) A: H1299 7.5% SDS PAGE GTX110237 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX110237 WB Image
GALNS antibody detects GALNS protein by western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg C2C12 whole cell lysate/extract 7.5% SDS-PAGE GALNS antibody (GTX110237) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX110237 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using GALNS(GTX110237) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
GTX110237 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GALNS(GTX110237) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.
|
文献引用
产品评论
目前没有使用 GALNS antibody (GTX110237) 的产品评论被收录。快发表第一篇产品评论吧!