产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:20000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
NIH-3T3 , mouse brain , PC-12 , rat brain , coli M15 , 293T , A431 , HeLa , HepG2
预测种属反应
Rabbit, Sheep, Bovine, Cat, Chicken, Guinea pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_11174761
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP
细胞定位
Cytoplasm , perinuclear region , Membrane
功能与背景
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX627408 WB Image
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis. A. 30 μg yeast whole cell lysate/extract 10% SDS-PAGE GAPDH antibody [GT239] (GTX627408) dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 WB Image
Sample (30 μg of whole cell lysate) A: Drosophila 10% SDS PAGE GTX627408 diluted at 1:5000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 ICC/IF Image
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: GAPDH stained by GAPDH antibody [GT239] (GTX627408) diluted at 1:500.Blue: Hoechst 33342 staining.
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GTX627408 WB Image
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 IHC-P Image
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.GAPDH stained by GAPDH antibody [GT239] (GTX627408) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX627408 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 WB Image
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg NIH-3T3 whole cell lysate/extract C. 30 μg mouse brain lysate/extract D. 30 μg PC-12 whole cell lysate/extract E. 30 μg rat brain lysate/extract 10% SDS-PAGE GAPDH antibody [GT239] (GTX627408) dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 WB Image
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis. A. 20 μg 293T whole cell lysate/extract B. 10 μg 293T whole cell lysate/extract C. 5 μg 293T whole cell lysate/extract D. 1 μg 293T whole cell lysate/extract 10% SDS-PAGE GAPDH antibody [GT239] (GTX627408) dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
GTX627408 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
GTX627408 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
GTX627408 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
GTX627408 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 WB Image
The data was published in the journal Oncotarget in 2015.PMID: 26327325
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GTX627408 WB Image
The data was published in the Nat Commun in 2020. PMID: 33257668
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GTX627408 WB Image
The data was published in the journal Front Physiol in 2019. PMID: 31681015
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GTX627408 WB Image
The data was published in the journal Biomed Res Int in 2015. PMID: 26448933
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GTX627408 WB Image
The data was published in the journal Biomed Res Int in 2015. PMID: 26448933
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GTX627408 WB Image
The data was published in the journal PLoS One in 2016. PMID: 27832139
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GTX627408 WB Image
The data was published in the 2022 in Sci Rep. PMID: 35484165
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GTX627408 WB Image
The data was published in the 2022 in Pathogens. PMID: 35335638
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GTX627408 WB Image
The data was published in the 2022 in Sci Rep. PMID: 35484165
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GTX627408 WB Image
The data was published in the 2022 in Pathogens. PMID: 35335638
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GTX627408 WB Image
The data was published in the 2020 in Cell Commun Signal. PMID: 33228717
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GTX627408 WB Image
The data was published in the 2020 in Cell Commun Signal. PMID: 33228717
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GTX627408 WB Image
The data was published in the 2020 in Cell Commun Signal. PMID: 33228717
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GTX627408 WB Image
The data was published in the J Cell Mol Med in 2020. PMID: 32827244
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GTX627408 WB Image
The data was published in the J Cell Mol Med in 2020. PMID: 32827244
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GTX627408 WB Image
The data was published in the journal J Biol Chem in 2016.PMID: 26742848
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2015.PMID: 26681405
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GTX627408 WB Image
The data was published in the journal EMBO Mol Med in 2013.PMID: 23828861
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2016.PMID: 27681590
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GTX627408 WB Image
The data was published in the journal J Biol Chem in 2016.PMID: 26742848
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GTX627408 WB Image
The data was published in the journal J Biol Chem in 2016.PMID: 26742848
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GTX627408 WB Image
The data was published in the journal J Biol Chem in 2016.PMID: 26742848
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GTX627408 WB Image
The data was published in the journal Front Neurol in 2019.PMID: 31258504
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GTX627408 WB Image
The data was published in the journal PLoS One in 2013.PMID: 24282540
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GTX627408 WB Image
The data was published in the journal J Cell Mol Med in 2015.PMID: 25683605
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GTX627408 WB Image
The data was published in the journal Nat Commun in 2019.PMID: 31337756
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2020.PMID: 32150830
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GTX627408 WB Image
The data was published in the journal Cell Death Dis in 2020.PMID: 32034133
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GTX627408 WB Image
The data was published in the journal Nat Commun in 2017.PMID: 28198375
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2018.PMID: 29933620
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GTX627408 WB Image
The data was published in the journal Nat Commun in 2018.PMID: 30030436
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GTX627408 WB Image
The data was published in the journal J Cancer Sci Ther in 2018.PMID: 29780443
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2017.PMID: 28747643
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2020.PMID: 32150830
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2015.PMID: 26681405
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2020.PMID: 32150830
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GTX627408 WB Image
The data was published in the journal Cell Death Dis in 2020.PMID: 32034133
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GTX627408 WB Image
The data was published in the journal Oncotarget in 2016.PMID: 27708250
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2018.PMID: 29933620
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2018.PMID: 29933620
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GTX627408 WB Image
The data was published in the journal Curr Biol in 2017.PMID: 28966089
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GTX627408 WB Image
The data was published in the journal J Biol Chem in 2019.PMID: 30898877
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GTX627408 WB Image
The data was published in the journal J Biol Chem in 2016.PMID: 26742848
|
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2015.PMID: 26681405
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GTX627408 WB Image
The data was published in the journal PLoS One in 2014.PMID: 24718614
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GTX627408 WB Image
The data was published in the journal J Biol Chem in 2016.PMID: 26742848
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2016.PMID: 27681590
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2018.PMID: 29933620
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2016.PMID: 27307030
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2015.PMID: 26681405
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2017.PMID: 29038428
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GTX627408 WB Image
The data was published in the journal Nat Commun in 2016.PMID: 27492621
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2015.PMID: 26681405
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GTX627408 WB Image
The data was published in the journal J Cancer Sci Ther in 2018.PMID: 29780443
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2018.PMID: 29933620
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2016.PMID: 27681590
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GTX627408 WB Image
The data was published in the journal Oxid Med Cell Longev in 2020.PMID: 32089770
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GTX627408 WB Image
The data was published in the journal PLoS One in 2013.PMID: 23977396
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GTX627408 WB Image
The data was published in the journal Front Neurol in 2019.PMID: 31258504
|
|
GTX627408 WB Image
The data was published in the journal Oncotarget in 2018.PMID: 29581854
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GTX627408 WB Image
The data was published in the 2020 in Cell Commun Signal. PMID: 33228717
|
|
GTX627408 WB Image
The data was published in the 2022 in Int J Mol Sci. PMID: 35054778
|
|
GTX627408 WB Image
The data was published in the 2020 in Cell Commun Signal. PMID: 33228717
|
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GTX627408 WB Image
The data was published in the 2020 in PLoS One. PMID: 32163428
|
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GTX627408 WB Image
The data was published in the 2022 in Nat Commun. PMID: 35618735
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GTX627408 WB Image
The data was published in the 2016 in PLoS Genet. PMID: 27727273
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GTX627408 WB Image
The data was published in the 2022 in Nat Commun. PMID: 35618735
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GTX627408 WB Image
The data was published in the 2022 in Nat Commun. PMID: 35618735
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GTX627408 WB Image
The data was published in the 2022 in EMBO Mol Med. PMID: 35253392
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GTX627408 WB Image
The data was published in the 2022 in Front Aging Neurosci. PMID: 35264943
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GTX627408 WB Image
The data was published in the 2022 in Front Aging Neurosci. PMID: 35264943
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GTX627408 WB Image
The data was published in the 2016 in PLoS Genet. PMID: 27727273
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GTX627408 WB Image
The data was published in the journal PLoS One in 2014. PMID: 25166596
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GTX627408 WB Image
The data was published in the journal Nucleic Acids Res in 2013. PMID: 23658231
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GTX627408 WB Image
The data was published in the journal PLoS One in 2014. PMID: 25166596
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GTX627408 WB Image
The data was published in the journal PLoS One in 2014. PMID: 25166596
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GTX627408 WB Image
The data was published in the journal PLoS One in 2014. PMID: 25166596
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GTX627408 WB Image
The data was published in the journal Cell Death Dis in 2017. PMID: 29238067
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GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799
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GTX627408 WB Image
The data was published in the journal Oncogene in 2017. PMID: 28319065
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2017. PMID: 29273797
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GTX627408 WB Image
The data was published in the journal Biomed Res Int in 2014. PMID: 25162007
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GTX627408 WB Image
The data was published in the journal Nat Commun in 2017. PMID: 28134239
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GTX627408 WB Image
The data was published in the journal Nat Commun in 2017. PMID: 28134239
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GTX627408 WB Image
The data was published in the journal eNeuro in 2017. PMID: 28856243
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GTX627408 WB Image
The data was published in the journal PLoS One in 2018. PMID: 29444136
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2014. PMID: 24406502
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GTX627408 WB Image
The data was published in the journal Mol Oncol in 2017. PMID: 28100038
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2017. PMID: 28829370
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2017. PMID: 28829370
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GTX627408 WB Image
The data was published in the journal ASN Neuro in 2013.PMID: 24286475
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2016.PMID: 27222304
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GTX627408 WB Image
The data was published in the journal J Biol Chem in 2016.PMID: 26742848
|
|
GTX627408 WB Image
The data was published in the journal Front Neurol in 2019.PMID: 31258504
|
|
GTX627408 WB Image
The data was published in the journal J Biol Chem in 2016.PMID: 26742848
|
|
GTX627408 WB Image
The data was published in the journal Oxid Med Cell Longev in 2020.PMID: 32089770
|
|
GTX627408 WB Image
The data was published in the journal PLoS One in 2018. PMID: 29444136
|
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2017. PMID: 28157211
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GTX627408 WB Image
The data was published in the journal J Virol in 2017. PMID: 28679762
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GTX627408 WB Image
The data was published in the journal J Virol in 2017. PMID: 28679762
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GTX627408 WB Image
The data was published in the journal Biomed Res Int in 2014. PMID: 25162007
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GTX627408 WB Image
The data was published in the journal Commun Biol in 2019. PMID: 31754649
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GTX627408 WB Image
The data was published in the journal Biomed Res Int in 2014. PMID: 25162007
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GTX627408 WB Image
The data was published in the journal Biomed Res Int in 2014. PMID: 25162007
|
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GTX627408 WB Image
The data was published in the 2021 in Nat Commun. PMID: 33712616
|
|
GTX627408 WB Image
The data was published in the J Cell Mol Med in 2020. PMID: 32827244
|
|
GTX627408 WB Image
The data was published in the J Cell Mol Med in 2020. PMID: 32827244
|
|
GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31311187
|
|
GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799
|
|
GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31311187
|
|
GTX627408 WB Image
The data was published in the journal Cell Death Dis in 2017. PMID: 29238067
|
|
GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31311187
|
|
GTX627408 WB Image
The data was published in the journal Cells in 2019. PMID: 31766533
|
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GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31311187
|
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GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799
|
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GTX627408 WB Image
The data was published in the journal Acta Neuropathol Commun in 2019. PMID: 30626447
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2015. PMID: 26151845
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GTX627408 WB Image
The data was published in the journal Acta Neuropathol Commun in 2019. PMID: 30626447
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2015. PMID: 26151845
|
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GTX627408 WB Image
The data was published in the journal Acta Neuropathol Commun in 2019. PMID: 30626447
|
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GTX627408 WB Image
The data was published in the journal Acta Neuropathol Commun in 2019. PMID: 30626447
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GTX627408 WB Image
The data was published in the journal Cardiovasc Diabetol in 2018. PMID: 29903013
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GTX627408 WB Image
The data was published in the journal Front Cell Neurosci in 2014. PMID: 25071443
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GTX627408 WB Image
The data was published in the journal Front Cell Neurosci in 2014. PMID: 25071443
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GTX627408 WB Image
The data was published in the journal Front Cell Neurosci in 2014. PMID: 25071443
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GTX627408 WB Image
The data was published in the journal Acta Neuropathol in 2018. PMID: 28780615
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GTX627408 WB Image
The data was published in the journal Acta Neuropathol in 2018. PMID: 28780615
|
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GTX627408 WB Image
The data was published in the journal Nat Commun in 2016. PMID: 27460411
|
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GTX627408 WB Image
The data was published in the journal Acta Neuropathol in 2018. PMID: 28780615
|
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GTX627408 WB Image
The data was published in the journal PLoS One in 2015. PMID: 26110811
|
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GTX627408 WB Image
The data was published in the journal Oncogene in 2017. PMID: 28319065
|
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GTX627408 WB Image
The data was published in the journal Hum Mol Genet in 2015. PMID: 26085575
|
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GTX627408 WB Image
The data was published in the journal Cell Death Dis in 2017. PMID: 29238067
|
|
GTX627408 WB Image
The data was published in the journal Oncogene in 2017. PMID: 28319065
|
|
GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2020. PMID: 31947604
|
|
GTX627408 WB Image
The data was published in the journal Cell Death Dis in 2017. PMID: 29238067
|
|
GTX627408 WB Image
The data was published in the journal Cells in 2019. PMID: 31766533
|
|
GTX627408 WB Image
The data was published in the journal Cardiovasc Diabetol in 2018. PMID: 29903013
|
|
GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799
|
|
GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2019. PMID: 31323961
|
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2019. PMID: 31323961
|
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GTX627408 WB Image
The data was published in the journal Cells in 2019. PMID: 31640218
|
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GTX627408 WB Image
The data was published in the journal Cells in 2019. PMID: 31766533
|
|
GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799
|
|
GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31311187
|
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GTX627408 WB Image
The data was published in the journal Molecules in 2017. PMID: 28531143
|
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GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799
|
|
GTX627408 WB Image
The data was published in the journal Oncotarget in 2017. PMID: 27926533
|
|
GTX627408 WB Image
The data was published in the journal Oncogene in 2017. PMID: 28319065
|
文献引用
产品评论
GAPDH antibody [GT239]
目录号 GTX627408
Rating
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( Average 4.5 based on 6 users reviews)
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实验应用
Western Blot(WB)
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( Average 4.5 based on 6 users reviews)
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Date :
Anonymous submitted on 24-Apr-2017
Application Tested :
WB
Sample Species :
Rat
Sample :
Brain tissue
Amount used :
50 μg
Blocking :
3% BSA in TBST, 23°C, 1Hr
Primary Antibody :
1:10000, 4°C, 16Hr
Date :
Anonymous submitted on 30-Jun-2014
Application Tested :
WB
Sample Species :
Hu
Sample :
KATOIII cells, MKN45 cells
Amount used :
100μg
Blocking :
skim milk, 25°C, 1Hr
Primary Antibody :
1:5000, 4°C, 16Hr
条带说明 :
Lane1:KATOIII cells, Lane2:MKN45 cells
Date :
Anonymous submitted on 18-Apr-2014
Application Tested :
WB
Sample Species :
Hu
Sample :
SHSY-5Y cells
Amount used :
30μg
Blocking :
5% Skim milk in TBST, 25°C, 2Hr
Primary Antibody :
1:5000, 4°C, 16Hr
Date :
Anonymous submitted on 06-Mar-2014
Application Tested :
WB
Sample Species :
Rat
Sample :
PC12 cells
Amount used :
20μg
Blocking :
5% nonfat milk, 22°C, 0.5Hr
Primary Antibody :
1:1000, 22°C, 2Hr
Figure Note :
starreivew courtesy of Xiaobei(Laboratory of UCSD)
Date :
Anonymous submitted on 06-Feb-2012
Application Tested :
WB
Sample Species :
Hu
Sample :
293T cells
Amount used :
30μg
Blocking :
TBST with 5% milk, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 06-Feb-2012
Application Tested :
WB
Sample Species :
Hu
Sample :
M10 cells
Amount used :
40μg
Blocking :
TBST with 5% milk, 25°C, 1Hr
Primary Antibody :
1:5000, 25°C, 1Hr