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GATA4抗体

Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194
Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194
Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113194
Anti-GATA4 antibody used in Western Blot (WB). GTX113194
Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194
Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194
Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113194
Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194
Anti-GATA4 antibody used in Western Blot (WB). GTX113194

目录号 GTX113194

文献引用 6
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 GATA4 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

45 kDa. ( Note )

预测种属反应

Rat, Bovine, Dog, Chicken, Pig, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human GATA4. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10726138

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

GATA binding protein 4 , ASD2 , TACHD , TOF , VSD1

细胞定位

Nucleus

功能与背景

This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194

GTX113194 IHC-P Image

GATA4 antibody detects GATA4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E13.5 embryo.
Green: GATA4 stained by GATA4 antibody (GTX113194) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194

GTX113194 IHC-P Image

GATA4 antibody detects GATA4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
GATA4 stained by GATA4 antibody (GTX113194) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113194

GTX113194 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GATA4(GTX113194) antibody at 1:500 dilution.

Anti-GATA4 antibody used in Western Blot (WB). GTX113194

GTX113194 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GATA4 antibody (GTX113194) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194

GTX113194 IHC-P Image

GATA4 antibody detects GATA4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
GATA4 stained by GATA4 antibody (GTX113194) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194

GTX113194 IHC-P Image

GATA4 antibody detects GATA4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
GATA4 stained by GATA4 antibody (GTX113194) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113194

GTX113194 ICC/IF Image

GATA4 antibody detects GATA4 protein at nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GATA4 stained by GATA4 antibody (GTX113194) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194

GTX113194 IHC-P Image

The data was published in the journal Sci Rep in 2017. PMID: 28102204

Anti-GATA4 antibody used in Western Blot (WB). GTX113194

GTX113194 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28102204

文献引用

Application Reference

CH Wang et al. Regen Ther 2023; 24 : 443-450 Cbx7 promotes the generation of induced pluripotent stem cells
实验应用 : ICC/IF
Lee YH et al. Theranostics 2019; 9 (22) : 6443-6465 Role of FOXC1 in regulating APSCs self-renewal via STI-1/PrPC signaling.
实验应用 : ICC/IF
种属反应 : Human
Abdelmonem M et al. Biomed Pharmacother 2019; 112 : 108584 Hydrogen sulfide enhances the effectiveness of mesenchymal stem cell therapy in rats with heart failure: In vitro preconditioning versus in vivo co-delivery.
Lao S et al. Mol Med Rep 2017; 16 (1) : 507-514 A comparative study of the influence of two types of PHEMA stents on the differentiation of ASCs to myocardial cells.
实验应用 : WB
种属反应 : Human
Qian Y et al. J Transl Med 2017; 15 (1) : 69 Multiple gene variations contributed to congenital heart disease via GATA family transcriptional regulation.
实验应用 : WB
种属反应 : Human
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产品评论

GATA4 antibody 目录号 GTX113194

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实验应用
Western Blot(WB)
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Anti-GATA4 antibody used in Western Blot (WB). GTX113194
Date : Anonymous submitted on 15-Apr-2014
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse heart tissue
Amount used : 8μg
Blocking : 3% milk in PBST, 23°C, 1Hr
Primary Antibody : 1:500, 4°C, 20Hr
我要评论产品
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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