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GATA4抗体

Orthogonal Overexpression
Anti-GATA4 antibody used in Flow cytometry (FACS). GTX54530
Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX54530
Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530
Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530
Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530
Anti-GATA4 antibody used in Western Blot (WB). GTX54530
Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX54530
Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530
Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530
Anti-GATA4 antibody used in Flow cytometry (FACS). GTX54530
Anti-GATA4 antibody used in Flow cytometry (FACS). GTX54530
Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX54530
Anti-GATA4 antibody used in Western Blot (WB). GTX54530

目录号 GTX54530

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, FACS

种属反应

 Human, Mouse
产品包装
100 μg (¥5300)
查看所有 GATA4 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:100-1:1000
FACS 3-5 μg/10⁶ cells
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

45 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 1mg/ml BSA, 30% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

Pooled peptides: RKEGIQTRKRKPKNLNKSK and CKQDSWNSLVLADSHGDIITA

纯化方式

Purified by antigen-affinity chromatography

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

GATA binding protein 4 , ASD2 , TACHD , TOF , VSD1

细胞定位

Nucleus

功能与背景

This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

数据库

研究领域

產品图片

Anti-GATA4 antibody used in Flow cytometry (FACS). GTX54530

GTX54530 FACS Image

FACS analysis of HepG2 cells using GTX54530 GATA4 antibody compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX54530

GTX54530 IHC-P Image

IHC-P analysis of human small intestine using GTX54530 GATA4 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200

Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530

GTX54530 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX54530 GATA4 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530

GTX54530 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX54530 GATA4 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530

GTX54530 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54530 GATA4 antibody.
Green : Primary antibody
Blue : cell nucleus
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for 1 hour at room temperature

Anti-GATA4 antibody used in Western Blot (WB). GTX54530

GTX54530 WB Image

WB analysis of whole cell extracts (20 μg lysate) of SH-SY5Y (Lane 1) and Hep G2 (Lane 2) using GTX54530 GATA4 antibody.
Dilution : 1:500-1:2000

Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX54530

GTX54530 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX54530 GATA4 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200

Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530

GTX54530 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54530 GATA4 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54530

GTX54530 ICC/IF Image

ICC/IF analysis of permeabilized embryoid body endoderm generated from human episomal iPSC using GTX54530 GATA4 antibody.
Green : Primary antibody
Blue : cell nucleus
Dilution : 1:100 in 3% BSA/PBS blocking buffer overnight at 4ºC

Anti-GATA4 antibody used in Flow cytometry (FACS). GTX54530

GTX54530 FACS Image

FACS analysis of Hela cells using GTX54530 GATA4 antibody compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

Anti-GATA4 antibody used in Flow cytometry (FACS). GTX54530

GTX54530 FACS Image

FACS analysis of NIH/3T3 cells using GTX54530 GATA4 antibody compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX54530

GTX54530 IHC-P Image

IHC-P analysis of mouse pancreas tissue using GTX54530 GATA4 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:500

Anti-GATA4 antibody used in Western Blot (WB). GTX54530

GTX54530 WB Image

Orthogonal Overexpression

WB analysis of 40 ug of HeLa nuclear fraction (NF) (Lane 1), HEK293T control (Lane 2), and HEK293T GATA4 over-expression lysate (Lane 3) using GTX54530 GATA4 antibody.
Dilution : 1:1000

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 5300
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