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GFAP抗体, C-term

Anti-GFAP antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89226
Anti-GFAP antibody, C-term used in Western Blot (WB). GTX89226
Anti-GFAP antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89226
Anti-GFAP antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89226
Anti-GFAP antibody, C-term used in Western Blot (WB). GTX89226
Anti-GFAP antibody, C-term used in ELISA (ELISA). GTX89226

目录号 GTX89226

宿主

Goat

克隆

Polyclonal

同种型

IgG

实验应用

WB, IHC-P, IHC-Fr, ELISA, Sandwich ELISA

种属反应

Human, Mouse, Rat
产品包装
100 μg (¥5600)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 0.003-0.03μg/ml
IHC-P Assay dependent
IHC-Fr Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

50 kDa. ( Note )

阳性对照

The peptide used to generate this antibody is available for purchase (GTX89226-PEP).

预测种属反应

Dog(>80% identity)

产品属性

形式

Liquid

存储溶液

TBS, 0.5% BSA

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Peptide with sequence C-DGEVIKESKQEHKD, from the C Terminus of the protein sequence according to NP_002046.1.

纯化方式

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

glial fibrillary acidic protein , ALXDRD

细胞定位

Cytoplasm

功能与背景

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

数据库

研究领域

產品图片

Anti-GFAP antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89226

GTX89226 IHC-P Image

IHC-P analysis of human cerebellum using GTX89226 GFAP antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml

Anti-GFAP antibody, C-term used in Western Blot (WB). GTX89226

GTX89226 WB Image

WB analysis of mouse (Lane A) and rat (Lane B) brain lysate (35μg protein in RIPA buffer) using GTX89226 GFAP antibody, C-term.
Dilution : 0.003μg/ml

Dilution : 0.003μg/ml

Anti-GFAP antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89226

GTX89226 IHC-P Image

IHC-P analysis of human cortex using GTX89226 GFAP antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

Anti-GFAP antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89226

GTX89226 IHC-Fr Image

IHC-Fr analysis of PFA-perfused human hypothalamus using GTX89226 GFAP antibody, C-term.
Antigen retrieval : citrate buffer pH 6 at 80C for 30min
Dilution : 0.01μg/ml

Anti-GFAP antibody, C-term used in Western Blot (WB). GTX89226

GTX89226 WB Image

WB analysis of human cerebellum (Lane A) and cerebral cortex (Lane B) lysate (35μg protein in RIPA buffer) using GTX89226 GFAP antibody, C-term.
Dilution : 0.001μg/ml

Dilution : 0.001μg/ml

Anti-GFAP antibody, C-term used in ELISA (ELISA). GTX89226

GTX89226 ELISA Image

ELISA analysis of GFAP protein using GTX89226 GFAP antibody, C-term.
Capture : GTX108711 (5μg/ml)
Detection : GTX89226 (5μg/ml)

文献引用

产品评论

GFAP antibody, C-term 目录号 GTX89226

Rating ( Average 5 based on 1 users reviews)
实验应用
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 02-May-2024
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : mouse primary astrocytes
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.2% Triton X-100 in PBS, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note :
Red : GFAP (GTX89226)
Blue : DAPI
SDS
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 5600
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