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GFP抗体 (AP)

Anti-GFP antibody (AP) used in Western Blot (WB). GTX26661
Anti-GFP antibody (AP) used in Dot blot (Dot). GTX26661
Anti-GFP antibody (AP) used in Western Blot (WB). GTX26661
Anti-GFP antibody (AP) used in IHC (Frozen sections) (IHC-Fr). GTX26661

目录号 GTX26661

文献引用 1

宿主

 Goat

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-Fr, Dot, ELISA, IHC

种属反应

 Species independent
产品包装
100 μg (¥4700)
查看所有 GFP 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:2500
ICC/IF 1:200-1:1000
IHC-Fr Assay dependent
Dot Assay dependent
ELISA 1:2000-1:8000
IHC 1:200-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品说明

No reaction was observed against Human, Mouse and Rat Serum Proteins.

产品属性

形式

Liquid

存储溶液

50mM Tris-HCl, 150mM NaCl, 1mM MgCl₂, 0.1mM ZnCl₂, 1% BSA, 50% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Jellyfish

免疫原

Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

纯化方式

Purified by antigen-affinity chromatography.
From serum

偶联

Alkaline Phosphatase (AP)

RRID

AB_371411

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

功能与背景

Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation.

研究领域

產品图片

Anti-GFP antibody (AP) used in Western Blot (WB). GTX26661

GTX26661 WB Image

Western Blot of Alkaline Phosphatase conjugated Goat anti-GFP antibody (GTX26661). Lane 1: GFP. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Block for 30 min RT. Predicted/Observed size: 28 kDa for GFP. Other band(s): none

Anti-GFP antibody (AP) used in Dot blot (Dot). GTX26661

GTX26661 Dot Image

Dot blot analysis of GFP using GTX26661 GFP antibody (AP).
Lane 1 : 100 ng
Lane 2 : 33.33 ng
Lane 3 : 11.11 ng
Lane 4 : 3.7 ng
Lane 5 : 1.23 ng
Dilution : 1:1000

Anti-GFP antibody (AP) used in Western Blot (WB). GTX26661

GTX26661 WB Image

WB analysis of recombinant GFP protein using GTX26661 GFP antibody (AP).
Loading : 50 ng
Dilution : 1:1000

Anti-GFP antibody (AP) used in IHC (Frozen sections) (IHC-Fr). GTX26661

GTX26661 IHC-Fr Image

The data was published in the journal BMC Biotechnol in 2013.PMID: 24073829

文献引用

Application Reference

Pavoni E et al. BMC Biotechnol 2013; 13 : 79 Simultaneous display of two large proteins on the head and tail of bacteriophage lambda.
实验应用 : ELISA
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SDS
Glycerol.pdf
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 4700
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