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GFP抗体 (Biotin)

Anti-GFP antibody (Biotin) used in Western Blot (WB). GTX26658

目录号 GTX26658

文献引用 12

宿主

 Goat

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, ELISA, IHC

种属反应

 Species independent
产品包装
100 μg (¥4300)
查看所有 GFP 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:2000 - 1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA 1:50000 - 1:80000
IHC 1:1000 - 1:5000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品属性

形式

Liquid

存储溶液

0.02M Potassium Phosphate, 0.15M NaCl, 1% BSA

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

纯化方式

Purified by antigen-affinity chromatography.
From polyclonal serum.

偶联

Biotin
Ratio : 10-20 Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) molecules per Goat IgG molecule.

RRID

AB_371422

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

功能与背景

Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation

研究领域

產品图片

Anti-GFP antibody (Biotin) used in Western Blot (WB). GTX26658

GTX26658 WB Image

Western blot of Biotin Goat Anti-GFP antibody. Lane 1: GFP. Load: 50 ng per lane. Primary antibody: GFP antibody Biotin conjugated at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for GFP. Other band(s): None.

文献引用

Application Reference

Bettke JA et al. Infect Immun 2022; 90 (4) : e0007022 Inflammatory Monocytes Promote Granuloma-Mediated Control of Persistent Salmonella Infection.
实验应用 : IHC-P
Franziska A Graef et al. PLoS Pathog 2021; 17 (8) : e1009719 Fasting increases microbiome-based colonization resistance and reduces host inflammatory responses during an enteric bacterial infection.
实验应用 : IHC-P
Manko-Prykhoda A et al. Int J Parasitol 2020; 50 (4) : 263-275 Giardia spp. promote the production of antimicrobial peptides and attenuate disease severity induced by attaching and effacing enteropathogens via the induction of the NLRP3 inflammasome.
实验应用 : IHC-P
Schmitt M et al. Cell Rep 2018; 24 (9) : 2312-2328.e7 Paneth Cells Respond to Inflammation and Contribute to Tissue Regeneration by Acquiring Stem-like Features through SCF/c-Kit Signaling.
实验应用 : WB
Dunlap MD et al. Mucosal Immunol 2018; (Epub) A novel role for C-C motif chemokine receptor 2 during infection with hypervirulent Mycobacterium tuberculosis.
实验应用 : IHC-P
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Application Reference
Bettke JA et al. Infect Immun 2022; 90 (4):e0007022 Inflammatory Monocytes Promote Granuloma-Mediated Control of Persistent Salmonella Infection.
实验应用 : IHC-P
Franziska A Graef et al. PLoS Pathog 2021; 17 (8):e1009719 Fasting increases microbiome-based colonization resistance and reduces host inflammatory responses during an enteric bacterial infection.
实验应用 : IHC-P
Manko-Prykhoda A et al. Int J Parasitol 2020; 50 (4):263-275 Giardia spp. promote the production of antimicrobial peptides and attenuate disease severity induced by attaching and effacing enteropathogens via the induction of the NLRP3 inflammasome.
实验应用 : IHC-P
Schmitt M et al. Cell Rep 2018; 24 (9):2312-2328.e7 Paneth Cells Respond to Inflammation and Contribute to Tissue Regeneration by Acquiring Stem-like Features through SCF/c-Kit Signaling.
实验应用 : WB
Dunlap MD et al. Mucosal Immunol 2018; (Epub) A novel role for C-C motif chemokine receptor 2 during infection with hypervirulent Mycobacterium tuberculosis.
实验应用 : IHC-P
Houbracken I et al. Sci Rep 2017; 7 (1):12643 Acinar cells in the neonatal pancreas grow by self-duplication and not by neogenesis from duct cells.
Manko A et al. PLoS One 2017; 12 (6):e0178647 Giardia co-infection promotes the secretion of antimicrobial peptides beta-defensin 2 and trefoil factor 3 and attenuates attaching and effacing bacteria-induced intestinal disease.
实验应用 : IHC-P
Paratore F et al. Anal Chem 2017; (Epub) Isotachophoresis-Based Surface Immunoassay.
Morampudi V et al. Infect Immun 2016; (Epub) Tricellular Tight Junction Protein Tricellulin is targeted by the Enteropathogenic E. coli Effector EspG1 Leading to Epithelial Barrier Disruption.
实验应用 : ICC/IF
Song I et al. PLoS One 2015; 10 (10):e0140148 Beta Cell Mass Restoration in Alloxan-Diabetic Mice Treated with EGF and Gastrin.
Burger NB et al. Prenat Diagn 2014; Involvement of neurons and retinoic acid in lymphatic development: new insights in increased nuchal translucency.
Bergstrom KS et al. PLoS Pathog 2010; 6 (5):e1000902 Muc2 protects against lethal infectious colitis by disassociating pathogenic and commensal bacteria from the colonic mucosa.
实验应用 : IHC-P
SDS
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 4300
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