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GFP抗体 (HRP)

Anti-GFP antibody (HRP) used in Western Blot (WB). GTX26663
Anti-GFP antibody (HRP) used in Western Blot (WB). GTX26663

目录号 GTX26663

宿主

Goat

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IP, ELISA, IHC, Multiplexing

种属反应

Species independent
产品包装
100 μg (¥4300)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:2000-1:5000
ICC/IF Assay dependent
IP Assay dependent
ELISA 1:10000-1:50000
IHC 1:500-1:2000
Multiplexing Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品说明

No reaction was observed against Human, Mouse and Rat Serum Proteins.

产品属性

形式

Liquid

存储溶液

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Jellyfish

免疫原

Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

纯化方式

Purified by antigen-affinity chromatography.
From serum

偶联

Horseradish peroxidase(HRP)

RRID

AB_371425

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

功能与背景

Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.

研究领域

產品图片

Anti-GFP antibody (HRP) used in Western Blot (WB). GTX26663

GTX26663 WB Image

Western Blot of Anti-GFP Antibody Peroxidase Conjugate (GTX26663). Lane 1: 50ng of GFP. Primary antibody: none. Secondary antibody: Anti-GFP Antibody Peroxidase Conjugate secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS 30 min at 20ºC. Predicted/Observed size: 28 kDa for GFP. Other band(s): none.

Anti-GFP antibody (HRP) used in Western Blot (WB). GTX26663

GTX26663 WB Image

WB analysis of various samples using GTX26663 GFP antibody (HRP).
Lane 1 and 14 : Protein ladder
Lane 2 : 293T whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : CHO-K1 whole cell lysate
Lane 5 : C2C12 whole cell lysate
Lane 6 : NIH-3T3 whole cell lysate
Lane 7 : Mouse embryonic fibroblast lysate
Lane 8 : HCP
Lane 9 : MBP
Lane 10 : RFP
Lane 11 : GFP
Lane 12 : GST
Lane 13 : MBP
Dilution : 1:1000

文献引用

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产品包装 报价 (¥)
¥ 4300
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